摘要
目的探讨雷公藤内酯醇对糖尿病大鼠心肌内葡萄糖转运体(Glut)-1、Glut-4表达的干预。方法雄性SD大鼠60只,按体质量随机分为正常对照组10只和糖尿病模型组50只。糖尿病模型组一次性腹腔注射链脲佐菌素53 mg/kg,正常对照组仅腹腔注射等体积0.1 mol/L柠檬酸缓冲液。注射链脲佐菌素72 h后,尾静脉采血测空腹血糖并同时测尿糖,血糖≥16.7 mmol/L、尿糖(+++)以上者列入糖尿病观察对象。将造模成功的48只雄性大鼠,按体质量、空腹血糖随机分为糖尿病模型对照组(12只),雷公藤内酯醇小、中、大剂量组(各12只)。结果雷公藤内酯醇中、大剂量组大鼠心体比值均比糖尿病对照组降低,但都高于正常对照组;雷公藤内酯醇中、大剂量组大鼠心肌中Glut-1、Glut-4的表达均比糖尿病对照组有显著增高,但都较正常对照组明显减少;雷公藤内酯醇小剂量组糖尿病大鼠心体比值、心肌中Glut-1、Glut-4的表达与糖尿病对照组相比变化不明显。结论雷公藤内酯醇能明显上调糖尿病大鼠心肌细胞内Glut-1、Glut-4的表达,改善高血糖对糖尿病大鼠心肌的毒性作用。
Objective To discuss intervention of triptolide on glucose transporter type （Glut）-1, Glut-4 expres-sion in the myocardium of diabetic rats. Methods Sixty SD rats were randomly divided into normal control group （n=10） and diabetes model （DM）group （n=50）. Diabetic model group were injected of streptozotocin （STZ） 53 mg/kg intraperitoneally, the normal control group were injected volume of the buffer. After the injection of STZ 72 h , fasting glucose and simultaneously measuring urine were tested from tail vein blood , both blood glucose≥16.7 mmol/L and rather than urine （＋＋＋） were included in the DM observed object. The successful model of 48 male rats , according to body weight, fasting blood glucose, DM model were randomly divided into a control group （12）, triptolide （TP） of small, medium and large-dose group （12 each）. Results TP in medium （1.8 g/kg） and high dose （2.2 g/kg） of rats heart body ratio were lower than DM group significantly, but were higher than the normal control group; the expression of TP in medium and high dosage groups in myocardium of rats with Glut-1 and Glut-4 were significantly higher than DM group. But all decreased significantly comparing with normal group; TP group of small dose of diabetic rat heart body ratio, myocardial Glut-1, Glut-4 expression comparing with the DM group did not change significantly. Conclusion Triptolide significantly increases Glut-1 and Glut-4 expression in diabetic rat myocardial cells, improves the toxic effect of hyper-glycemia on myocardium of DM rats.
出处
《实用医技杂志》
2015年第6期573-575,共3页
Journal of Practical Medical Techniques
基金
河南省教育厅自然科学研究计划项目(2011C310003)
