摘要
目的：研究葡萄内酯对小鼠离体小肠的作用机制。方法：采用小鼠离体小肠平滑肌实验,研究葡萄内酯对正常小鼠离体小肠、抑制剂（致痉剂）诱导的离体小肠的影响。结果：表明葡萄内酯（1.75×10-7~1.4×10-5mol·L-1）对正常小鼠离体小肠收缩有明显的兴奋作用,随剂量增大兴奋作用增强;当剂量大于1.75×10-5mol·L-1,其作用转为抑制。葡萄内酯对普萘洛尔、茶苯海明、阿托品、氯解磷定、肾上腺素诱导的小肠舒张各有不同程度的拮抗作用并能显著增强乙酰胆碱诱导的小肠痉挛作用。结论：葡萄内酯具有拟胆碱作用,低浓度下可促进小肠收缩运动,高浓度可致小肠收缩痉挛,可成为ACh E抑制剂。
Objective： To investigate the effect of auraptene on contraction of isolated intestinal smooth muscle from mice and the mechanism. Methods： In isolated intestinal smooth muscle from mice, the effect of au- raptene was evaluated in the absence or presence of inhibitors and spasmogens. Results： Auraptene accelerated small intestinal contraction in a concentration-dependent manner at 1.75 × 10-7 1.4 × 10-5 mol· L-1, but had an inhibition at ≥ 1.75 × 10-s mol· L-1. Auraptene antagonized intestinal relaxation induced by propranolol, dimen- hydrinate, atropine, pyraloxime chloride and adrenaline at different degrees. Furthermore, auraptene markedly en- hanced small intestinal spasm induced by acetylcholine. Conclusion： Auraptene at low concentrations can promote the intestinal contraction, but high concentrations can cause small intestinal spasm. Auraptene has a cholinergic effect, so it may be an AChE inhibitor.
出处
《中国新药杂志》
CAS
CSCD
北大核心
2015年第12期1404-1407,共4页
Chinese Journal of New Drugs
基金
国家自然科学基金(81260612
81460575)
江西省青年科学基金(20122BAB215039)
江西省自然科学基金(20132BAB205092)
关键词
葡萄内酯
小肠收缩
离体小肠
抑制剂
致痉剂
auraptene
intestinal contraction
isolated intestinal smooth muscle
inhibitors
spasmogens
