摘要
目的 观察关节囊切开与未切开内固定复位对兔创伤性股骨颈骨折的影响.方法 将60只健康雄性新西兰大白兔随机分为3组：假手术组（S组,8只）、关节囊切开复位组（O组,26只）和关节囊未切开复位组（C组,26只）,采用手术结合钝性打击的方法制作股骨颈骨折模型,内固定复位;X线检查股骨颈骨折愈合,酶联免疫吸附试验（ELISA）、免疫磁珠技术检测术后外周血中骨钙素（BGP）水平和循环内皮细胞（CEC）数量,苏木素-伊红（HE）染色后镜下观察股骨头组织形态的变化并统计空骨陷窝率.结果 术后8、12周X线评分,O组分别为（31.6±4.8）、（34.4±7.6）分,高于C组（23.4±3.1）、（29.0±5.9）分（P＜0.05）;BGP水平和CEC数量,O、C组均较S组升高（P＜0.05）,O组和C组比较,差异无统计学意义（P＞0.05）;S、C、O组空骨陷窝率分别为（2.4±1.1）％、（23.7±3.3）％和（11.6±4.8）％,C组较O组升高（P＜0.05）.结论 兔创伤性股骨颈骨折后行关节囊切开内固定复位有利于骨折愈合,并可降低股骨头坏死率.
Objective To observe the therapeutic effect of treating traumatic femoral neck fracture rabbits with open or close joint capsule reduction.Methods Sixty healthy male New Zealand white rabbits were randomly divided into 3 groups：sham-operated group （S group,n =8）,close restoration group （C group,n =26） and open restoration group （O group,n =26）.The animal models of traumatic femoral neck fracture were established in O and C groups.A ＂T＂ type of incision was made in the joint capsue in O group,and the joint capsule in C group was not damaged.The curative effectiveness of femoral neck fractures was evaluated by X-ray examination.The bone gla protein （BGP） expression was assayed in serum by enzyme linked immunosorbent assay （ELISA）,and circulating endothelial cells （CECs） were tested by immunomagnetic beads.The femoral head and neck of the rabbits killed at 12th week after operation were observated histologically.Results The X-ray scores and 8th and 12th week in O group （31.6 ±4.8 and 34.4 ± 7.6） were significantly higher than in C group （23.4 ± 3.1 and 29.0 ± 5.9） （P ＜ 0.05）.The BGP expression and number of CECs in O group and C group were significantly increased as compared with S group （P ＜ 0.05）,but there was no significant difference between C group and O group （P ＞ 0.05）.Histologically,the rate of empty bone lacuna in O group was significantly lower than in C group [（11.6 ± 4.8） ％ vs.（23.7 ± 3.3） ％,P ＜ 0.05].Conclusion After traumatic femoral neck fracture,the open joint capsule reduction method can reduce the pressure of joint capsule and protect the residual blood supply,which is conducive to fracture healing.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2015年第6期1381-1383,共3页
Chinese Journal of Experimental Surgery
基金
武汉市卫生局临床医学科研项目（WX13B12）
关键词
股骨颈骨折
关节囊切开
内固定复位术
Femoral neck fracture
Open joint capsule
Reduction and internal fixation
