摘要
目的探讨石杉碱甲（huperzine A，HupA）预处理对老年大鼠七氟醚麻醉后认知功能的影响。方法24只健康sD大鼠，采用随机数字表法分为两组：对照组（c组），HupA组（H组），每组12只。H组老年大鼠每日1次经口灌胃0．15mg／kg剂量的HupA，C组经口灌胃等量蒸馏水，两组大鼠均吸入3％七氟醚100min／d，连续5d。采用Morris水迷宫实验及旷场试验测定大鼠认知功能，记录逃避潜伏期、穿越平台次数、跨格次数、直立次数及中央格停留时间。结果H组老年大鼠逃避潜伏期较c组缩短，穿越平台次数[（2．3±0．8）次]较C组[（2．0±0．5）次]增多，差异均有统计学意义（P〈0．05）；H组老年大鼠跨格次数[（66±18）次]较C组[（38±9）次]增多，中央格停留时间[（15±4）s]较C组[（35±8）s]明显减少，直立次数[（8．2±2．1）次]较C组[（4．0±1．3）次]明显增多，差异均有统计学意义（P〈0．05）。结论HupA预处理可以改善老年大鼠七氟醚麻醉后认知功能。
Objective To investigate the effects of huperzine A（Hup A） preconditioning on cognitive function in elderly rats after sevoflurane anesthesia. Methods Twenty-four SD rats aged 22 months were randomly divided into 2 groups （n=12）： control group（group C）, Hup A group（group H）. The group H was given oral garage 0.15 mg/kg Hup A with 0.01 ml/g weight, group C was given oral garage distilled water. Group C and group H exposed to 3% sevoflurane in oxygen （2.5 L/min） and nitrogen （6 L/min） 100 min/d for 5 consecutive days respectively. Cognitive function was detected using Moms water-maze and Open-field test. The escape latency, the number of cross the platform location, the number of cross-grid, the number of uptight and the number of central cell residence time were recorded. Results Compared with group C, the escape latency was shortened and the number of cross the platform location （2.3±0.8 ） was increased in group H, the difference was statistically significant （P〈0.05）. The number of cross-grid （66±18） increased significantly and the number of uptight （8.2±2.1） were increased significantly and the number of central cell residence time [ （15±4） s] was prolonged in groups H than in group C, the differences were significant （P〈0.05）. Conclusions Huperzine A preconditioning can improve the cognitive function in elderly rats after sevoflurane anesthesia.
出处
《国际麻醉学与复苏杂志》
CAS
2015年第6期485-487,共3页
International Journal of Anesthesiology and Resuscitation
基金
河南省医学科技攻关计划重点项目（201002020）
关键词
石杉碱甲
老年
大鼠
七氟醚
认知功能
Huperzine A
Aged
Rat
Sevoflurane
Cognitive function
