摘要
运用荧光光谱、红外光谱、圆二色谱和拉曼光谱等四种光谱手段,研究了鱼胶原蛋白肽(FCP)与表没食子儿茶素没食子酸酯(EGCG)在水溶液中的相互作用。荧光结果表明:EGCG使FCP中的酪氨酸荧光强度减小,促进了二酪氨酸的形成;FCP与EGCG能够形成FCP-EGCG非共价复合物,同时,EGCG影响了FCP中酪氨酸的微环境。红外光谱分析表明:FCP具有胶原蛋白特征吸收带;EGCG的加入使3 281cm-1处的吸收峰消失,3 076cm-1处的吸收峰红移,表明EGCG影响了酰胺A带和酰胺B带;1 659和1 689cm-1处的吸收峰蓝移,1 547cm-1处的吸收峰红移以及1 248cm-1处吸收峰的消失,表明FCP中酰胺Ⅰ带、酰胺Ⅱ带和酰胺Ⅲ带均受到EGCG的影响。圆二色谱分析表明:添加EGCG后,FCP 222nm处的负峰消失,198nm处的负峰依次红移至200和204nm,说明EGCG影响了FCP的二级结构。拉曼光谱分析结果表明:EGCG的加入影响了FCP中酰胺Ⅰ带、酰胺Ⅱ带和酰胺Ⅲ带的吸收;同时,EGCG的添加使863和932cm-1处的峰红移,前者峰强度降低,后者峰强度大幅增加,表明羟脯氨酸和脯氨酸均参与了与EGCG的结合,且EGCG浓度的增加使更多的脯氨酸暴漏。
Fish collagen is known to have good moisturising property and antioxidant ability ,which has been increasingly added into cosmetics ,foods and drinks as thicker agent and to increase dietary supply of collagen .Fish collagen peptide （FCP） is a white or pale yellow powder ,obtained by extracting collagen from sources including the scales and bones of fish such as bonito , halibut ,tuna ,and sea bream .It is identical to human collagen and 100% absorbable through the skin .（-）-Epigallocatechin-3-gallate （EGCG） ,the major constituent of green tea ,has lots of beneficial biological and pharmacological effects ,including an-tioxidant ,antimutagenic ,antiviral and antiinflammatory activities .Because proteins have the desirable formulation of EGCG-for-tified food ,the interaction between proteins and EGCG molecules has been widely studied .At the same time ,the interaction of proteins and EGCG was known to affect the content of free EGCG ,structure of proteins ,antioxidant capacity of EGCG in foods . But ,to our knowledge ,the interaction between FCP and EGCG has not been characterised clearly ,and little is known about their interaction mechanism .Therefore ,a better understanding of the interaction between FCP and EGCG would help to control their functional properties in food products during processing ,transportation and storage when we facilitate FCP as the vehicles for EGCG .In view of the above ,we planned to study the interaction of FCP with EGCG by using different spectroscopic tech-niques ,such as fluorescence spectroscopic ,FTIR ,CD and Raman .EGCG caused a concentration dependent quenching of the in-trinsic fluorescence of tyrosine residue in the FCP ,indicating the occurrence of interactions between FCP and EGCG .Excimer-like species and dityrosine were regularly formed with the addition of EGCG into the solution ,and the interaction of FCP and EGCG partly disrupted the structure of the protein .Synchronous fluorescence results indicate that the interaction caused de-crease of the polarity aroun
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
2015年第1期184-188,共5页
Spectroscopy and Spectral Analysis
基金
国家自然科学基金项目(31371835)资助
