摘要
目的研究子宫内膜癌患者血清多肽谱的变化,探讨子宫内膜癌早期诊断相关的差异蛋白。方法选取2008-05-01-2011-05-31浙江省肿瘤医院收治的子宫内膜癌患者134例、子宫体良性病变患者103例和健康女性志愿者95名作为研究对象。将研究对象分为测试组(45例Ⅰ期子宫内膜癌患者和45名健康女性)、验证组(134例子宫内膜癌患者和95名健康女性),采用基体辅助激光解吸电离飞行时间质谱(matrix-associated laser disso-ciation/ionization time of flight mass spectrmtry,MALDI-TOF-MS)结合纳米磁珠技术测定其血清多肽谱,Biomarker Wizard软件筛选差异蛋白峰,采用SPSS 12.0中ROC曲线分析差异峰建立诊断模型,进一步评价模型对诊断子宫内膜癌的敏感度和特异性,并分析差异蛋白峰在各期子宫内膜癌及子宫良性病变患者中的分布情况。结果血清多肽谱检测结果显示,测试组质荷比为1 500~30 000m/z,共测得82个蛋白峰,其中有12个差异峰差异有统计学意义,P〈0.001。在12个差异峰中,取ROC曲线面积≥0.8的2 768、3 402和6 441m/z 3个差异峰作为子宫内膜癌的诊断模型,其敏感度分别为78.4%、75.4%和66.8%,特异性分别为92.6%、89.5%和86.2%;三者联合的敏感度和特异性为88.1%和75.8%。联合诊断模型鉴别子宫内膜癌和子宫良性病变的敏感度为94.9%。结论血清多肽谱检测筛查发现的差异峰2 768、3 402和6 441m/z能较正确的区分子宫内膜癌患者、健康人和子宫良性病变患者,有助于子宫内膜癌的早期筛查。
OBJECTIVE To identify serum biomarkers related to early endometrial cancer.METHODS Serum proteins or peptides were purified with weak cation exchange magnetic beads in 134 patiens with endometrial cancer,95 healthy women,and 103 patients with benign uterine diseases.Distinct peaks were selected using Biomarker Wizard software.The area under receiver operating characteristic curve(AUC)was generated to analyze discrimination capability of peaks between endometrial cancer,health and benign diseases.RESULTS Twelve distinct peaks at the molecular range of 1 500-30 000m/z were identified between 45 early endometrial cancer and 45 health people(P〈0.001).There were3peaks(2 768,3 402 and 6 441 m/z)with AUC greater than 0.8.A diagnostic model consisting of 3protein peaks at2 768,3 402 and 6 441m/z was established,while their sensitivity were 78.4%,75.4%and 66.8%,and their specificity were 92.6%,89.5% and 86.2%,respectively.A sensitivity of 88.1% and a specificity of 75.8%in a large scale blind test between endometrial cancer and healthy person were obtained.The sensitivity of diagnostic model was 94.9% between endometrial cancer and benign diseases.CONCLUSION The diagnostic model consisting of 3protein peaks at2 768,3 402 and 6 441m/z can discriminate endometrial cancer patients from healthy women and benign disease patients.
出处
《中华肿瘤防治杂志》
CAS
北大核心
2015年第11期875-879,共5页
Chinese Journal of Cancer Prevention and Treatment