摘要
[目的]评价氰霜唑在葡萄和土壤中的安全性,对氰霜唑及其代谢物在葡萄和土壤中的残留消解动态进行研究。[方法]葡萄和土壤中的氰霜唑通过乙腈提取,二氯甲烷萃取,采用液相色谱串联质谱法检测（LC-MS/MS）。葡萄和土壤中的氰霜唑代谢物（CCIM）通过乙腈提取,采用液相色谱串联质谱法检测（LC-MS/MS）。[结果]氰霜唑在葡萄和土壤中的平均回收率为86.0%~107.9%和98.3%~104.6%,相对标准偏差为6.56%~7.29%和1.33%~6.43%。CCIM在葡萄和土壤中的平均回收率为82.5%~110.6%和75.9%~97.6%,相对标准偏差为3.16%~9.59%和7.67%~10.98%。氰霜唑及CCIM的最小检出量为0.1 ng,氰霜唑及CCIM在葡萄和土壤中的最低检测质量分数为0.01 mg/kg。氰霜唑及其代谢物之和在葡萄和土壤中的半衰期分别为7.27、11.49 d。[结论]该方法简单可靠,符合农药残留分析要求,可用于葡萄和土壤中氰霜唑的残留检测。氰霜唑在葡萄和土壤中均易降解。
[Aims] To evaluate the safety of cyazofamid in grape and soil, field experiments were conducted to study the residues and dissipation dynamics of cyazofamid and its metabolite（CCIM）. [Methods] Residues of cyazofamid in grape and soil were extracted with acetonilrile, and the extract was extracted by dichloromethane, analyzed by LC-MS/MS under multi-reaction monitoring （MRM） mode with electrospray ionization （positive mode）. CCIM in grape and soil were extracted with acetonilrile, and analyzed by LC-MS/MS under MRM mode. [Results] The average recoveries of cyazofamid in grape and soil were 86.0-107.9%, 98.3-104.6%, and the coefficient of variation were 6.56-7.29%, 1.33-6.43%, respectively. The average recoveries of CCIM in grape and soil were 82.5-110.6%, 75.9-97.6%, and the coefficient of variation were 3.16-9.59%, 7.67-10.98%, respectively. The minimum detectable quantity of cyazofamid and CCIM was 0.1 ng, the limit of detection was 0.01 mg/kg. The half-lives of cyazofamid and CCIM in grape were 7.27 d, and the half-lives in soil were 11.49 d. [Conclusions] The method is simple and reliable, meet with the requirement of pesticide residue analysis, and can be applied for determining cyazofamid residues in grape and soil. Cyazofamid and CCIM are non-persistent chemical compounds.
出处
《农药》
CAS
CSCD
北大核心
2015年第6期438-441,共4页
Agrochemicals
