摘要
目的观察鼠卵巢癌模型在顺铂化疗前后雌激素水平的变化,评估顺铂腹腔化疗对卵巢功能的影响。方法取8周龄裸鼠36只,随机分为空白对照组(A组,n=8)、肿瘤细胞接种后不化疗组(B组,n=12)、肿瘤细胞接种后静脉化疗组(C组,n=8)、肿瘤细胞接种后腹腔化疗组(D组,n=8)。所有裸鼠均行一侧附件切除术,除A组外,术中向其余3组鼠腹腔内注射卵巢癌细胞悬液。于术后第9天对A组和B组裸鼠腹腔内注入生理盐水,C组裸鼠尾静脉注射顺铂(20μg/g)+腹腔内注射生理盐水,D组裸鼠腹腔内注射顺铂(20μg/g)。第1次化疗后第0、10、20天分别取鼠尾静脉血测量雌激素水平。化疗22 d后重复第1次化疗方法 1次。于第2次化疗后第10、20及30天分别取鼠尾静脉血测量雌激素水平。结果腹腔内接种肿瘤细胞后B、C及D组裸鼠雌激素水平明显高于A组(P<0.05),A与B组的雌激素水平相对稳定,C和D组雌激素水平在化疗后显著降低,但C组高于D组(P<0.05),随着化疗后时间延长,C与D组裸鼠雌激素水平逐渐下降至A组水平(P>0.05)。结论顺铂化疗会损伤卵巢功能,腹腔化疗对卵巢皮质的损伤可能大于静脉化疗,随着化疗间隙期的延长卵巢功能可以得到恢复。
Objective To investigate effects of intraperitoneal chemotherapy with cisplatin on serum estrogen levels in mice ovarian carcinoma model. Methods Thirty six female BALB / c-nu nude mice of 8 weeks were randomized into four groups： mice in group A（ n = 8） served as blank controls; mice in group B（ n = 12） were inoculated with ovarian carcinoma HO-8910 cells; mice in group C（ n = 8）were inoculated with HO-8910 cells and received intravenous injection of 20 μg / g cisplatin at the 9th day after surgery; mice in group D（ n = 8） were inoculated with ovarian carcinoma cells and received intraperitoneal injection of 20 μg / g cisplatin. Blood samples were taken via caudal vein on d0,10,20 after chemotherapy and serum estrogen levels were measured. The chemotherapy was repeated on the22 ed day, and estrogen levels were measured on d10,20,30 after the second chemotherapy.Results With the inoculation of cancer cells,the estrogen levels were significantly increase in groups B,C,D,compared to that in group A（ P 〈0. 05）,then the estrogen levels were suppressed by chemotherapy in group C and D. The estrogen levels in group D were significantly lower than that in group C（ P 〈0. 05）. On d30 after second chemotherapy,the estrogen level of group A,C and D had no significant difference（ P 〉0. 05）. Conclusion Chemotherapy can suppress ovarian function,intraperitoneal chemotherapy may be more toxic than intravenous chemotherapy; meanwhile ovarian function may gradually recover after chemotherapy.
出处
《同济大学学报（医学版）》
CAS
2015年第2期16-19,共4页
Journal of Tongji University(Medical Science)
基金
上海市卫生和计划生育委员会基金(WSJ1204)
关键词
卵巢肿瘤
动物研究
腹腔化疗
顺铂
雌激素
小鼠
ovarian tumour animal experiment intraperitoneal chemotherapy cisplatin estrogen mouse
