摘要
目的探讨机体Th1/Th2细胞相关因子及免疫状态在肝癌发生及家族聚集性中的作用。方法选取150例调查对象,其中来自肝癌高发家族患者共75例,包括癌症患者30例(A组)和未患肝癌的调查对象45例(B组),来自无癌家族(C组)患者75例。血清Th1细胞相关因子水平的检测以干扰素-γ(interferon-γ,IFN-γ)为代表,血清Th2细胞相关因子水平的检测以白介素-10(interleukin-10,IL-10)为代表。通过酶联免疫吸附法(enzyme-linked immuno sorbent assay,ELISA)检测各组患者的血清Th1和Th2细胞相关因子水平。研究Th1/Th2两种细胞相关因子及免疫状态的作用及其临床意义。采用独立样本的t检验和方差分析进行组间数据比较,并用多元线性回归分析预测组间的相关性。结果 Th1/Th2细胞因子水平比较结果表明,肝癌高发家族癌症患者的Th1细胞因子水平显著高于无癌家族组,Th2细胞因子水平显著低于无癌家族组(P<0.05)。肝癌高发家族中未患肝癌成员的Th1/Th2细胞因子水平与无癌家族组患者无统计学差异(P>0.05)。结论肝癌高发家族中癌症患者的Th1/Th2平衡状态改变,向Th2方向漂移。肝癌高发家族中未患肝癌患者的Th1/Th2水平有向Th2漂移倾向,肝癌高发家族中未患肝癌患者的免疫功能异常可能是癌变前就存在的,而不是肝细胞癌变后逐渐形成的。
Objective To investigate the effect of Thl/Th2 eytokines and immune state on the occurrence and familial aggregation of hepatocellular carcinoma. Methods One hundred and fifty people were involved in this study. Seventy five were enrolled from high oc- currence families. Among them, 30 cancer patients （group A） and 45 normal patients （group B ） were defined. Seventy five members without cancer were defined as group C. The level of peripheral blood Thl type and Th2 type cytokines were detected by enzyme linked immunosorbent assay （ELISA）. IFN -3＇ and IL - 10 were representative for Thl and Th2 type cytokines, respectively. To find out the effect and the clinical significance of Thl/Th2 eytokines and immune state, pairwise comparisons were carried out by t -test and multiple comparisons were carried out by variance analysis. Results There was a Thl/Th2 serum cytokine imbalance profile in members of HCC high - occurrence family. The level of IFN - ~/was significantly lower in members of HCC high - occurrence family than group C. The lev- el of IL - 10 was higher in members of HCC high - occurrence family than group C. Conclusion There was a poor cellular immune state in HCC in the high occurrence families. Thl type cytokines was inhibited and Th2 type cytokines was enhanced, so more susceptible to HBV chronic infection. It might be the mechanism of HCC occurrence and familial aggregation. The balance of Thl / Th2 in cancer pa- tients changes （ shift to Th2） among high occurrence families. Meanwhile, the Thl / Th2 in normal people also found the tendency of shift to Th2 among high occurrence families. The immune dysfunction of normal people among high occurrence families may be exist before cancer growths, not developed after formation of HCC.
出处
《医学研究杂志》
2015年第5期77-81,共5页
Journal of Medical Research
基金
浙江省科技计划项目(2013C33224)
关键词
肝细胞癌
细胞因子
免疫状态
家族聚集
Hepatocellular carcinoma
Cytokines
Immune state
Familial aggregation
