摘要
背景:肺癌干细胞与肺癌的治疗和预后等紧密相关,通过对其表面标志物和致瘤性的研究,可以为临床诊治提供更多的参考依据。目的:探讨肺癌球体干细胞的富集方法及其致瘤性。方法:使用含有表皮细胞生长因子、胰岛素样生长因子1以及碱性成纤维细胞生长因子的无血清培养基对肺癌细胞进行诱导,采用免疫荧光检测诱导后细胞相关标志物的表达。小鼠皮下移植诱导后肺球体细胞,以了解其致瘤性。结果与结论:经无血清悬浮培养和诱导,肺癌细胞形成细胞球体,免疫荧光检测显示80%以上的球体细胞呈SP-C、CCSP以及OCT4染色阳性。小鼠皮下移植球体细胞后可形成肿瘤。结果表明,无血清悬浮培养肺癌细胞可增殖形成细胞球体,该细胞球体中肺癌干细胞高度富集并具有致瘤性。
BACKGROUND：Lung cancer stem cel s are tightly related to the treatment and prognosis of lung cancer. We can provide more references for clinical diagnosis and treatment of lung cancer through the study on the tumorigenicity and surface markers of lung cancer stem cel s. OBJECTIVE：To explore the enrichment methods for lung cancer stem cel s and cel ular tumorigenicity. METHODS：Lung cancer stem cel s were induced in serum-free culture medium containing epidermal growth factor, insulin-like growth factor 1, and basic fibroblast growth factor. Then, the expressions of related surface markers were detected using immunofluorescence method. After that, mice were implanted subcutaneous with lung cancer stem cel spheres to understand the tumorigenicity of lung cancer stem cel s. RESULTS AND CONCLUSION：Lung cancer stem cel s under serum-free induction and culture were changed to sphere-forming cel s, and the immunofluorescence detection showed that over 80%of sphere-forming cel s were positive for CCSP, SP-C and OCT4. After transplantation of sphere-forming cel s, the mice showed a high tumorigenicity. These findings indicate that sphere-forming cel s are formed after serum-free suspension culture of lung cancer stem cel s, which have a higher tumorigenicity.
出处
《中国组织工程研究》
CAS
北大核心
2015年第14期2167-2171,共5页
Chinese Journal of Tissue Engineering Research
关键词
干细胞
肿瘤干细胞
肺癌
细胞生长因子
球体
标志物
Lung Neoplasms
Neoplastic Stem Cells
Culture Media,Serum-Free
Insulin-Like Growth Factor I
Epidermal Growth Factor
