摘要
目的探讨乌司他丁对中暑(HS)大鼠肾脏损伤和氧化应激的影响。方法 SPF级Wistar雄性大鼠42只,随机分为正常对照组(n=6)、中暑组(HS组,n=18)和乌司他丁治疗组(UTI组,n=18)。HS组及UTI组置于仿真高温气候舱内复制HS模型,以大鼠收缩压峰值开始下降为模型成功标志,对照组置于23.0±0.2℃室温下。UTI组于制模前腹腔注射UTI 10万U/kg,每12h重复给药,其余两组大鼠均注射等量生理盐水。分别于造模0,6,24h时点采血并处死大鼠,以自动生化分析仪检测大鼠血清肌酐(Cr)、尿素氮(BUN)水平,比色法检测肾脏组织丙二醛(MDA)及超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GPx)活性等氧化应激指标。光镜及电镜观察造模24h后大鼠肾组织形态学变化。结果与对照组比较,HS及UTI组大鼠肾组织MDA含量于模型复制成功后0h显著升高(P<0.05),6h及24h有所下降,但仍显著高于对照组(P<0.05)。UTI组各时间点肾组织MDA含量均明显低于HS组(P<0.05)。HS组大鼠肾组织SOD和GPx活性于模型复制后6h显著低于对照组(P<0.05),UTI组与对照组比较无明显下降(P>0.05)。与HS组比较,UTI组6h及24h血清Cr、BUN水平下降(P<0.05),中暑后24h肾脏的病理损害明显减轻。结论乌司他丁可减轻重症中暑大鼠肾脏氧化应激水平,从而保护肾功能。
Objective To study the effects of ulinastatin on oxidative stress factors and renal function in rats with severe heatstroke. Methods Forty-two male SPF Wistar rats were randomly divided into normal control group（group C, n=6）, heat stroke group（HS group, n=18） and ulinastatin treatment group（UTI group, n=18）. Rats in the HS group and UT group were placed in an artificial climate chamber to induce HS, while rats of group C were kept at room temperature（23±0.2℃）. UT rats were given by intraperitoneal injection of ulinastatin before model reproduction in a dose of 100 thousand units/kg, and it was repeated every 12 h. The other in the rest two groups were injected with equal amount of normal saline. All the rats were sacrificed in batches at 0, 6 and 24 h after successful modeling. Before death, blood samples were collected to determine serum creatinine and urea nitrogen with automatic biochemical analyzer. Meanwhile the concentrations of renal superoxide dismutase（SOD）, glutathione peroxidase（GPx） and malondialdehyde（MDA） were determined with colorimetric method. Pathological changes in renal tissue of all animals were observed at 24 h after model replication with light and electron microscopy. Results Compared with rats in group C, the MDA levels at 0h after onset of heatstroke increased significantly（P〈0.05） in the renal tissue of HS and UTI group, and they were also significantly higher at 6 and 24 h although they began to fall afterward（P〈0.05）. The MDA level was significantly lower in the UTI group than that in the HS group at each time point（P〈0.05）. The levels of SOD and GPx in the rat kidney tissue of HS group were lower than that of group C at 6h after successful model replication（P〈0.05）, while there was no significant decline in UTI groupcompared with group C（P〉0.05）. At the same time, compared with HS group, the elevated levels of serum Cr and BUN in the UTI group were lower at 6 and 24 h after heatstroke onset（all P〈0.05）. The pathologi
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2015年第5期354-357,共4页
Medical Journal of Chinese People's Liberation Army
基金
中国博士后科学基金(2012M512181)
广东省医学科研基金(A2013439)~~
关键词
乌司他丁
热应激障碍
氧化性应激
中暑
肾功能不全
ulinastatin
heat stress disorders
oxidative stress
heatstroke
renal insufficiency
