摘要
目的探讨子宫颈上皮内瘤变(cervical intraepithelial neoplasia,CIN)与子宫颈癌组织中细胞周期抑制因子p16蛋白及特异识别病毒复制的中间产物TLR3的表达及意义。方法采用免疫组化SP两步法检测19例正常子宫颈、62例CIN及17例子宫颈鳞癌中p16和TLR3的表达。结果正常子宫颈、CIN及子宫颈鳞癌中p16的阳性率分别为0、72.5%、100%,各组间差异有统计学意义(P<0.01);TLR3阳性率分别为26.3%、87%、100%,各组间差异有统计学意义(P<0.01);p16及TLR3的表达呈显著正相关(rs=0.538,P<0.01)。结论 p16和TLR3在CIN及子宫颈癌组织中表达较正常鳞状上皮均显著提高,且p16及TLR3的表达与CIN级别相关,可为早期子宫颈癌的病理诊断提供实验依据。
Purpose To investigate the expression and clinical significance of the cell cycle inhibitors p16 protein and specific recogni-tion of viral replication intermediate TLR3 in the cervical intraepithelial neoplasia （ CIN） and cervical invasive carcinoma. Methods Immunohistochemical stain was used to detect the expressions of p16 and TLR3 in 19 cases of normal cervical epithelium （ NCE） , 62 cases of CIN, and 17 cases of cervical squamous cell carcinoma （SCC）. Results The positive rates of p16 protein were 0, 72. 5%and 100% in NCE, CIN and SCC respectively in which the difference among those groups were statistically significant （ P〈0. 01 ） . Similarly, the positive rates of TLR3 protein were 26. 3%, 87% and 100% in NCE, CIN and SCC respectively and the difference a-mong those groups was significant （P〈0. 01）. Furthermore, there was a significant and positive correlation between the expression of p16 and TLR3 （rs =0. 538, P〈0. 01）. Conclusion Increased expression is observed in CIN and SCC compared with NCE and the expression of p16 and TLR3 is associated with level of CIN. Those could provide certain experiment basis for the pathologica diagnosis of early cervical cancer.
出处
《临床与实验病理学杂志》
CAS
CSCD
北大核心
2015年第5期502-505,共4页
Chinese Journal of Clinical and Experimental Pathology
