摘要
家蚕核型多角体病毒(Bombyx mori nucleopolyhedrovirus,Bm NPV)病是世界养蚕业一种重大的传染疾病,该病引起的蚕茧损失占蚕病引起损失总数的60%以上,长期以来对这种病毒病的防治还没有很好的解决方法.各国研究人员在寻找抗性基因、阐述抗性机制以及通过传统育种或者转基因构建抗Bm NPV家蚕等方面进行了很好的尝试,也取得了一定的成果.本文将最新的家蚕抗Bm NPV的研究结果进行归纳和整理,尝试对其可能的分子机制进行探讨,并对现有的研究结果进行深入挖掘,希望有助于家蚕抗病分子机制的深入研究.
Bombyx mori nucleopolyhedrovirus （BmNPV） causes an important viral infectious disease in sericulture, and is responsible for 60% of the total cocoon production loss caused by various silkworm diseases. Horizontal resistance of silkworms to BmNPV is well known but we have identified a local line, named NB, whose LD50 is more than 1000 times higher than that of susceptible strains, and that has the highest resistance to BmNPV out of 344 silkworm lines. Through l0 years of crossbreeding and systematic selective breeding using line NB, we have constructed a commercial variety of Bombyx rnori that is highly resistant to BmNPV. These genetic studies indicate the existence of BmNPV resistance genes in silkworm, and a pair of dominant genes has been implicated. Recently, we constructed a BmNPV-resistant near-isogenic line （NIL） via interspecific hybridization and backcrossing. Using this model and molecular techniques, such as fluorescent differential display-PCR, suppression subtractive hybridization, DNA microarray and proteomic analyses, we have identified molecules involved in BmNPV resistance. This review summarizes the current research status of BmNPV resistance in silkworms and discusses molecules involved in anti-BmNPV processes.
出处
《科学通报》
EI
CAS
CSCD
北大核心
2015年第14期1285-1297,1-3,共13页
Chinese Science Bulletin
基金
国家重点基础研究发展计划(2012CB114604)
国家自然科学基金(31272507
31301919)
江苏省普通高校研究生科研创新计划(KYXX0010)资助
关键词
家蚕
核型多角体病毒
抗性
遗传分析
SSH
蛋白质组
免疫反应
Bombyx mori, nucleopolyhedrovirus, resistance, genetic analysis, SSH, proteomic, immune response
