摘要
目的探讨年青人骨髓间充质干细胞(h MSCs)来源生物活性因子对年老人h MSCs功能的影响。方法低氧无血清培养条件下获得年青及年老h MSCs条件培养基(CM)。将年老h MSCs置于年青h MSCs来源CM中,或相反将年青h MSCs置于年老h MSCs来源CM中。通过Transwell实验、油红O染色、茜素红染色、β半乳糖苷酶染色、qRT-PCR分别检测不同来源CM中生物活性因子对h MSCs迁移能力、向脂肪细胞及成骨细胞分化能力、衰老、microRNA表达的影响。结果年青h MSCs来源CM生物活性因子可促进年老h MSCs功能再生,增强迁移能力和向脂肪细胞及成骨细胞分化能力,减少衰老。相反,将年青h MSCs置于年老h MSCs来源CM中,年青h MSCs功能受损,迁移能力下降,向脂肪细胞及成骨细胞分化能力下降,衰老增加。qRT-PCR检测发现,年青h MSCs来源CM生物活性因子可促进h MSCs中miR-10a表达,而年老h MSCs来源CM生物活性因子可抑制其表达。结论年青h MSCs来源生物活性因子可促进miR-10a表达,促进h MSCs迁移、分化,减少细胞衰老,促进年老h MSCs功能再生。
Objective To investigate the effects of human mesenchymal stem cells（ h MSCs） derived bioactive factors on h MSCs function.Methods Old h MSCs were exposed to a young cell derived condition medium（ CM）.Conversely,young h MSCs were exposed to an old cell derived CM.The transwell tests were used to detect the migration capability of h MSCs.The differentiation capability was compared by inducing h MSCs into adipogenic and osteogenic cells.The effect of condition medium bioactive factors on h MSCsenescence were detected by β-galactosidase staining.The changes in microRNA（ miRNA） expression were determined by quantitative real-time PCR（ qRT-PCR）.Results Old h MSCs can be rejuvenated by exposure to a young cell derived CM.The h MSCmigration and differentiation ability was increased,and senescence was decreased.Conversely,the migration and differentiation ability of young h MSCs was impaired by exposure to an old cell derived CM.And the senescence was increased.The expression of miR-10 a was down-regulated in the old h MSCs derived CM,but was up-regulated in the young h MSCs derived CM.Conclusion Young cell derived bioactive factors can rejuvenate old h MSC function by increasing the expression of miR-10 a.
出处
《广东医学》
CAS
北大核心
2015年第10期1501-1504,共4页
Guangdong Medical Journal
基金
国家自然科学基金资助项目(编号:81401156)
广州市医药卫生科技项目(编号:20141A011085)
关键词
人骨髓间充质干细胞
年龄
细胞迁移
分化
human mesenchymal stem cells
aging
cell migration
differentiation
