摘要
目的 :研究胃癌中mi R-148a与E-钙黏蛋白(E-cadherin)表达的相关性,并进一步探索mi R-148a对E-cadherin的内在调控机制。方法:通过q RT-PCR或Western blot检测胃癌组织中E-cadherin及mi R-148a的表达,使用Pearson相关分析检测E-cadherin与mi R-148a表达的相关性;通过去甲基化药物处理,研究启动子异常甲基化与E-cadherin表达的关系;通过转染mi R-148a mimics提高mi R-148a的表达水平,转染DNA化转移酶1(DNMT1)si RNA干扰DNMT1的表达水平,进一步研究mi R-148a对E-cadherin的调控机制。结果 :与正常胃黏膜相比,E-cadherin的m RNA及蛋白表达水平在胃癌组织中显著下调,并与mi R-148a的表达呈正相关。去甲基化药物5-aza-d C处理后,胃癌细胞MGC-803及SGC-7901中E-cadherin的m RNA及蛋白表达水平显著上升。胃癌细胞转染mi R-148a mimics后,E-cadherin的蛋白表达水平明显提高,并且干扰DNMT1的表达后,Ecadherin的蛋白表达水平也显著上调。结论 :mi R-148a能通过DNMT1进一步调控E-cadherin的表达。
Objective：To investigate the correlation between E-cadherin and miR-148a,and explore the underlying mechanism of miR-148a regulating E-cadherin expression in gastric cancer. Methods：The mRNA levels of E-cadherin and miR-148a were detected by qRT-PCR, and protein expression of E-cadherin was assayed by Western blot;A correlation between E-cadherin mRNA levels and that of miR-148a in gastric cancer was investigated by Pearson correlation analyses;The relationship between aberrant methylation and E-cadherin expression was evaluated by treatment of gastric cancer cells with demethylation drug;The regulatory mechanism of miR-148a modulating E-cadherin was analyzed by transfection of gastric cancer cells with miR-148a mimics or DNA methyltransferase 1 （DNMT1） siRNA. Results：The mRNA and protein levels of E-cadherin were significantly downregulated in gastric cancer tissues compared with corresponding normal tissues, and were positively correlated with miR-148a expression. After treatment of MGC-803 and SGC-7901 cells with 5-aza-dC, a significant increase in E-cadherin mRNA and protein levels was observed. Moreover, overexpression of miR-148a,whieh had been verified to modulate DNMT1 expression, could reactivate the expression of E-cadherln. Knockdown of DNMT1 by DNMT1 siRNA could also increase the mRNA and protein levels of E-cadherin. Conclusion：The enforced expression of miR-148a may contribute to the reactivation of E-cadherin by suppression of DNMT1.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2015年第4期470-474,共5页
Journal of Nanjing Medical University(Natural Sciences)
基金
江苏省卫生厅科研立项(H201347)
无锡市医管中心联合攻关项目(YGZXY1317)
