摘要
目的研究金莲花提取工艺。方法采用正交试验法对金莲花中总黄酮进行提取,以出膏率和紫外分光光度法测定的总黄酮得率为指标,考察乙醇浓度(A)、回流时间(B、回流次数(C)、料液比(D)4个因素。检测波长为500 nm。结果优化的金莲花提取条件为A2B1C3D2。结论采用65%乙醇,料液比为1∶10,回流3次,每次2小时为最佳工艺,其中回流次数为显著性因素。黄酮最高含量为15.74%,最高出膏率为45.72%。
Objective to study on extraction process of Trollius Chinensis.Methods The study was carried out through Orthogonal Design,the congtent of Orientin determined by UV-spectrophotometry and the rate of obtaining paste were as guide.4 influencial factors,ethanol concentration（A）,time of extraction（B）,return the number（C）and material liquid than（D）were studied.The detection wavelength was 500 nm. Results The optimal conditions for the extraction were A2B1C3D2.Conclusion Extract three times by 65% ethanol,2 hours for every time,the material liquid than was 1：10,and the notable factor was return the number. The highest content of total flavones was 15.74%,and the highest extract rate was 45.72%.
出处
《科技创新导报》
2015年第4期1-1,3,共2页
Science and Technology Innovation Herald
关键词
金莲花
总黄酮
紫外分光光度法
正交试验法
Trollius Chinensis
Total Flavones
UV-spectrophotometry
Orthogonal Design
