摘要
目的：探讨9项呼吸道病原抗体检测在急性气管‐支气管炎患儿诊断中的价值。方法选取清远市妇幼保健院门诊部和住院部急性气管‐支气管炎患儿269例，其中2～12月患儿67例，＞1～4岁患儿86例，大于4岁患儿116例，全部患儿采用间接免疫荧光法检测9项呼吸道病原 IgM 抗体，观察单项及多项病原抗体检测阳性的分布情况，并分析不同年龄段患儿9项呼吸道病原抗体阳性率的差异，评价其在指导临床抗菌药物应用中的价值。结果9项呼吸道病原抗体检测阳性率为52．42％，其中单项呼吸道病原抗体阳性率为44．61％，多项病原抗体检测阳性率为7．81％。肺炎支原体抗体阳性率为26．39％，明显高于乙型流感病毒抗体阳性率7．43％、肺炎衣原体抗体阳性率4．83％，嗜肺军团菌抗体阳性率4．09％，差异有统计学意义（P ＜0．05）。不同年龄段抗体阳性率比较差异均有统计学意义（P＜0．05）。61例肺炎支原体抗体、11例嗜肺军团菌抗体和5例 Q 热立克次体阳性患儿均应用阿奇霉素治疗有效，肺炎支原体对气管‐支气管炎的诊断灵敏度、特异度、Kappa 值和受试者操作特征曲线下面积明显高于乙型流感病毒和肺炎衣原体。结论9项呼吸道病原抗体检测在急性气管‐支气管炎患儿诊断中阳性率较高，在辅助急性气管‐支气管炎诊断和指导抗菌药物应用中具有重要的意义。
Objective To investigate the value of nine respiratory tract antibodies detection in the diagnosis of children with a‐cute tracheobronchitis .Methods A total of 269 cases with acute tracheobronchitis were selected in outpatient department of Mater‐nal and Child Care Hospital of Qingyuan ,including 67 children for 2 - 12 months ,86 children for 〉 1 - 4 years old ,116 children for more than 4 years ,nine respiratory pathogens IgM antibodies were detected by indirect immunofluorescence technique ,the single and combined positive rate of the nine respiratory pathogen antibodies were observed ,and the positive rates of nine respiratory pathogens antibodies of children at different ages were analyzed ,the clinical antibiotics application value was evaluated .Results The positive rate of nine detection of respiratory pathogens antibodies was 52 .42% ,the positive rate of individual respiratory patho‐gen antibody was 44 .61% ,the positive rate of various pathogenic antibody detection was 7 .81% .The positive rate of mycoplasma pneumoniae antibody was 26 .39% ,which was significant higher than 7 .43% of influenza B virus antibody ,4 .83% of chlamydia pneumoniae antibody ,and 4 .09% of legionella pneumophila antibody ,the differences were significant （P〈 0 .05） .The differences of antibody positive rates in different age groups were significant （P 〈 0 .05） .61 children with mycoplasma pneumoniae antibody ,11 children with legionella .pneumophila antibody and 5 children with Q rickettsia positive were treated by azithromycin effectively . The diagnostic sensitivity ,specificity ,Kappa value and under area of receiver operating characteristic curve of mycoplasma pneu‐moniae were significant higher than influenza B virus and chlamydia pneumoniae antibody .Conclusion The diagnosis positive rate of nine respiratory pathogenic antibodies detection to children with acute tracheobronchitis is high ,which has important significance in the auxiliary acute tracheobronchitis diagnosis and guiding the antibiotic a
出处
《国际检验医学杂志》
CAS
2015年第11期1597-1598,1608,共3页
International Journal of Laboratory Medicine
关键词
呼吸道病原抗体
急性气管-支气管炎
上呼吸道感染
respiratory pathogenic antibody
acute tracheobronchitis
upper respiratory infection
