摘要
以垦芸2号芸豆品种为原料,以自来水和纯净水作对比试验,研究小分子团磁化水促进芸豆清蛋白的浸出作用,并优化了提取技术参数。通过对提取温度、提取时间、液料比和粉碎粒度四个因素的单因素试验和响应面分析,结果表明:提取温度31℃,提取时间2 h,液料比22∶1,粉碎粒度60目时芸豆清蛋白的提取率均最高,其中小分子团磁化水组提取率为48.96%,高于自来水组(42.79%)和纯净水组(40.87%);并对小分子团磁化水提取的芸豆清蛋白进行SDS-PAGE电泳测试,得出分子量排布主要集中在42.01 k Da左右,说明所得蛋白为清蛋白。
Using Kenyun 2 kidney beans as raw material and tap water and pure water as comparison tests,the infusion effects of magnetized water with small molecules mass to kidney bean were studied,and the extraction parameters of albumin from kidney bean were optimized. Through single factor and response surface analysis of four factors such as extraction temperature,extraction time, ratio of liquid to material and particle size,the results showed that the optimum extraction conditions was as follows：extraction temperature 31 ℃,extraction time 2 h,ratio of liquid to material 22∶1,particle size 60 mesh. Under such conditions,the extraction yield of magnetized water with small molecules group was 48.96% and higher than that of tap water group （42.79%）and purified water group （40.87%）.SDS-PAGE result showed that the molecular weight of the extracted protein was about 42.01 kDa,and the protein was albumin.
出处
《黑龙江八一农垦大学学报》
2015年第3期57-65,87,共10页
journal of heilongjiang bayi agricultural university
基金
国家杂粮工程技术研究中心组建项目(2011FU125X07)
关键词
芸豆清蛋白
小分子团磁化水
提取
SDS-PAGE
kidney bean albumin
magnetized water with small molecules mass
extraction yield
SDS-PAGE
