摘要
目的:鉴定小鼠胃癌移植模型中生成的肿瘤类型及病理特征。方法:采用HE染色法对肿瘤病理组织学特征进行观察,用免疫组织化学的方法分析肿瘤中CD43、CD45RB、CD57、CK等标志物的表达及小鼠脾脏中CD43、CD57的表达以诊断肿瘤类型及来源。结果:经组织学观察,小鼠皮下肿瘤组织与人胃癌组织结构形态并不相符。经免疫组织化学分析,小鼠肿瘤抗人体的CD43、CD45RB、CD57均为阳性,提示肿瘤细胞中由大量T淋巴细胞、B淋巴细胞和NK细胞组成;但小鼠脾脏内T、B淋巴细胞和NK细胞均为阴性。结论:小鼠皮下肿瘤为淋巴瘤,来源于移植的胃癌组织中带有的淋巴组织。
Objective：hTe tumor type and the pathological features of tumor were identiifed in murine xenogratfs model of gastric cancer. Methods：hTe tumor histopathologic features was observed by HE stainings;the expression of CD43, CD45RB, CD57, CK in mouse tumor and the expression of CD43 and CD57 in mouse spleen were analyzed by immunohistochemical method to diagnose the tumor types and sources. Results：The histological features and characteristics of the mouse subcutaneous tumor were not consistent with human gastric cancer by histological observation. hTe CD43, CD45RB, CD57 markers were positive in mouse tumor by immunohistochemical analysis, indicating that mouse tumor cells were constituted by T lymphocytes, B lymphocytes and NK cells. But in the mouse spleen, T lymphocytes, B lymphocytes and NK cells were all negative. Conclusion：hTe subcutaneous tumors of the NOD-SCID mice were lymphoma and derived from lymphoid tissue in gastric cancer by transplantation.
出处
《临床与病理杂志》
CAS
2015年第5期756-761,共6页
Journal of Clinical and Pathological Research
基金
武汉市科技计划(201260538027)
光谷3551人才计划~~
