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盐酸异丙肾上腺素诱发大鼠心肌缺血性纤维化后肝脏损伤的拉曼光谱分析 被引量:1

Analysis of Raman spectroscopy for Iso-induced liver injury after myocardial fibrosis of ischemia in rats
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摘要 目的:探讨盐酸异丙肾上腺素(Iso)诱发大鼠心肌缺血性纤维化后拉曼光谱对肝脏损伤的分析,为拉曼光谱的应用提供依据。方法:20只Wistar大鼠随机分为对照组、Iso-4h模型组、Iso-24h模型组和Iso-1周模型组(n=5);除对照组外,其余各组大鼠均一次性皮下多点注射Iso。HE染色分析心脏和肝脏的病理学改变;紫外光谱和拉曼光谱分析肝脏组织的蛋白吸收谱。结果:病理学观察,对照组大鼠心肌细胞排列整齐,横纹清晰,胞核明显,无细胞肿胀;Iso-24h组大鼠心肌出现较多的坏死,局限于心内膜下;Iso-1周组大鼠心肌出现界限清晰、多发散在坏死灶,坏死灶内有旺盛的成纤维细胞增生,并可见一定量的胶原纤维。对照组大鼠肝小叶界限清晰,无坏死灶;Iso各组大鼠肝小叶内有灶性坏死区,肝细胞出现水样变性,且随着Iso注射时间的延长,肝脏病变程度加重。紫外检测,与对照组比较,Iso-24h组大鼠心脏组织吸收峰向左或向右移动1个单位,Iso-1周组由1个峰位趋于正常;与对照组比较,Iso-24h组大鼠肝脏组织吸收峰移动了2个单位,Iso-1周组吸收峰位逐渐趋于平稳。拉曼光谱法,与对照组比较,Iso-24h组大鼠肝组织仅在1 590nm处见明显吸收峰,Iso-1周组未检测到相同吸收峰。结论:Iso诱发心肌损伤时,肝脏亦有损伤;拉曼光谱是更为灵敏的检测肝损伤的方法。 Objective To investigate the effect of Raman spectroscopy for isoprenaline hydrochloride(Iso)-induced liver injury after myocardial fibrosis of ischemia in the rats,and to provide the basis for the application of Raman spectroscopy.Methods 20 Wistar rats were randomly divided into control group,Iso-4hgroup,Iso-24 hgroup,and Iso-1week group(n= 5).Except the normal group,the rats in other groups were subcutaneously injected with Iso.The pathological changes of heart and liver tissues were analyzed by HE staining.The absorption spectra of proteins in the liver tissue was analyzed by UV and Raman spectroscopy.Results The pathological observation results showed that the cardiomyocytes of the rats in control group arranged in neat rows with clear stripes,obvious nucleus and no cell swelling;the cardiomyocytes of the rats in Iso-24 hgroup appeared the more necrosis limting under endocardium;the myocardium of the rats in Iso-1week group appeared the multiple scattered necrosis with fibroblasts and collagen fiber.The hepatic lobules of the rats in control group had clear boundaries without necrosis;the hepatic lobules of the rats in Iso groups had focal necrosis,and the liver cells appeared watery degeneration;with the prolongation of the Iso injection time,the degree of liver lesion was serious.The UV spectroscopy showed that the myocardial protein spectrum of the rats in Iso-24 hgroup moved 1unit to the left or the right,and the spectrum in Iso-1 week group became nromal compared with control group;the liver protein spectrum of the rats in Iso-24 hgroup moved 2units,and the spectrum in Iso-1week gradually stabilized compared with control group.The Raman spectrocopy showed that the absorption peak of liver tissue in Iso-24 hgroup appeared at 1 590 nm,but it wasn't found in Iso-1 week group.Conclusion Liver damage is found when Iso induces myocardium injury,and Raman spectroscopy is a more sensitive detection method for liver damage.
出处 《吉林大学学报(医学版)》 CAS CSCD 北大核心 2015年第3期584-587,F0003,共5页 Journal of Jilin University:Medicine Edition
基金 吉林省科技厅医药大品种专项资金资助课题(YYZX201259) 吉林省科技厅科学计划项目资助课题(201101092 20140312002ZG) 吉林大学基本科研费资助课题(409020720084)
关键词 盐酸异丙肾上腺素 心肌缺血 拉曼光谱法 肝脏 isoprenaline hydrochloride myocardial ischemia Raman spectroscopy liver
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  • 1RonaG, Chappel CI, Balazs T, et al. An infarct like myoeardiallesion and other toxic manifestations produced by isoproterenol in therat [J]. Arch Pathol, 1959, 67 (4): 443-455. 被引量:1
  • 2徐叔云,卞如濂,陈休.药理学实验方法[M].2版.北京:人民卫生出版社,1991:716-717. 被引量:7
  • 3朱彤莹,黄国英,顾勇,林善锬.两种心衰模型大鼠心功能的比较[J].中国实验动物学报,2002,10(1):51-53. 被引量:20
  • 4边长勇,秦永文,郑兴,章同华.大鼠充血性心力衰竭模型的制备[J].第二军医大学学报,2001,22(7):693-694. 被引量:2
  • 5Li W, Liang J, Yang W, et al. Chiral functionalization of graphene oxide by optically active helical-substituted polyacetylene chains and its application in enantioselective crystallization [J]. ACS App[ Mater Interfaces, 2014, 6 (12): 9790-9798. 被引量:1
  • 6Gupta N, Gupta D, Aggarwal S, et al. Thermally stable plasmonic nanocermets grown on microengineered surfaces as versatile surface enhanced Raman spectroscopy sensors for multianalyte detection [J]. ACS Appl Mater Interfaces, 2014, 6 (24): 22733-22742. 被引量:1
  • 7Lewis EN, Qi W, Kidder LH, et hi. Combined dynamic light scattering and Raman spectroscopy approach for characterizing the aggregation of therapeutic proteins [J]. Molecules, 2014, 19 (12): 20888-20905. 被引量:1
  • 8Szostak R, Mazurek S. Quantitative determination of acetylsalicylic acid and acetaminophen in tablets by FT-Raman spectroscopy [J]. Analyst, 2002, 127 (1): 144-148. 被引量:1
  • 9高海成,孙波,苗春生,高海梅,任立群.大剂量盐酸异丙肾上腺素诱发大鼠心肌缺血性坏死模型的建立[J].中国生物制品学杂志,2009,22(3):288-290. 被引量:14
  • 10Kasuya J, Tanishita K. Mieroporous membrane-based liver tissue engineering for the reconstruction of three-dimensionM functional liver tissues in vitro [ J ]. Biomatter, 2012, 2 (4): 290-295. 被引量:1

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