摘要
目的 ：动态检测食管癌患者放射治疗前后外周血mi R-21的表达水平并探讨其临床意义。方法 ：收集上海市第六人民医院肿瘤放疗科2009年8月-2013年6月食管癌首诊患者24例,健康志愿者19例作为对照组。应用实时荧光定量PCR法检测食管癌患者放射治疗前后以及健康志愿者外周血mi R-21水平,通过受试者工作特征（receiver operating characteristic,ROC）曲线的曲线下面积（area under the curve,AUC）评估外周血mi R-21对食管癌的诊断价值,分析mi R-21动态水平与食管癌患者临床病理特征、近期疗效以及生存期之间的关系。结果 ：食管癌患者放射治疗前mi R-21水平显著高于健康对照组（P〈0.01）,AU C值为0.8 9 5[9 5%可信区间（9 5%c o n f i d e n c e i n t e r v a l,9 5%C I）：0.797~0.992],外周血mi R-21水平作为食管癌诊断标志物的敏感度和特异度分别为91.67%和73.68%。食管癌患者放射治疗后mi R-21水平显著降低（P〈0.01）,且T3~T4期食管癌患者mi R-21水平较T1~T2期患者降低明显（P〈0.01）,食管鳞癌（esophageal squamous cell carcinoma,ESCC）患者较食管腺癌（esophageal adenocarcinoma,EAC）患者降低明显（P〈0.01）。外周血mi R-21水平与食管癌患者的生存期有关,放射治疗后mi R-21水平降低不明显的患者生存期较短（P〈0.05）。结论 ：外周血mi R-21水平可作为食管癌诊断及预测患者生存期的动态生物学检测指标。
Objective：To dynamically monitor the expression level of miR-21 in peripheral blood before and after radiotherapy in patients with esophageal carcinoma and explore its clinical implication.Methods：Twenty-four patients who were primarily diagnosed of esophageal carcinoma at Department of Radiation Oncology,Shanghai Sixth People＇s Hospital between August 2009 and June 201 3 were recruited,and simultaneously 19 healthy subjects were also recruited as the healthy controls.The miR-21 level in peripheral blood of patients with esophageal carcinoma before and after radiotherapy as well as the healthy subjects was examined by realtime fluorescence quantitative-PCR.The receiver-operating characteristic（ROC） curve and the area under the curve（AUC） were used to evaluate the accuracy of miR-21 level in peripheral blood predicting esophageal carcinoma.The relationships of change in miR-21 level with the clinicopathological characteristics,short-term response and the survival were analyzed.Results：The expression level of miR-21 before radiotherapy was significantly higher in patients with esophageal carcinoma than that in the healthy controls（P 0.01）,and the AUC value was 0.895[95%confidence interval（CI）：0.797-0.992].The sensitivity and specificity of miR-21 in predicting esophageal carcinoma were 91.67%and 73.68%,respectively.The expression level of miR-21 in patients with esophageal carcinoma after radiotherapy was significantly declined（P 0.01）.Furthermore,the expression level of miR-21 was declined significantly after radiotherapy in patients with phase T_（3-4） esophageal carcinoma as compared with that in patients with phase T_（1-2） esophageal carcinoma（P 0.01）;the expression levels of miR-21 was also declined significantly in patients with esophageal squamous-cell carcinoma（ESCC） as compared with that in patients with esophageal adenocarcinoma（EAC）（P 0.01）.The expression level of miR-21 in peripheral blood was associated with overall survival（OS） of patients with
出处
《肿瘤》
CAS
CSCD
北大核心
2015年第5期550-555,共6页
Tumor
关键词
食管肿瘤
微RNAS
聚合酶链反应
诊断
预后
Esophgeal neoplasms MicroRNAs Polymerase chain reaction Diagnosis Prognosis
