摘要
[目的]克隆决明丙二酰Co A:ACP转酰基酶(MCAT)基因并做序列分析。[方法]采用RACE法从c DNA中扩增出决明丙二酰Co A:ACP转酰基酶(MCAT)的CDS全长序列,推测出MCAT基因编码的氨基酸序列并进行序列分析。[结果]分析结果显示MCAT的CDS全长1 253bp,编码405个氨基酸。通过序列比对分析发现决明MCAT蛋白包含一个酰基转移酶超家族结构域,并且发现了一段高度保守的七肽模序。[结论]获得了决明MCAT的CDS全长,其编码的蛋白可能催化丙二酰Co A的转酰基反应,为决明脂肪酸的合成通路的阐明以及后期的决明脂肪酸的基因工程研究打下了坚实的基础。
[ Objective] To clone and sequence the cDNA encoding Malonyl - CoA transacylasegene of Cassia obtusifolia L. [ Methods] Ac- cording to the RACE method,the full -length CDS sequence of Malonyl -CoA transacylase （MCAT） of Cassia obtusifolia L. was obtained from the cDNA,deduced the amino acid sequence and analyzed by bioinformatics. [ Results]The results showed that the full -length cD- NA of MCAT had 1 253 bp and encoding 405 amino acids. Sequence aligenment showed that Cassia obtasifolia L. MCAT contained an acyl- transferase superfamily domain and a highly conserved motif. [ Conclusion] The full -length CDS of Cassia obtusifolia L. was obtained and the deduced protein was most like to catalyze the transacylation of malonyl - CoA. This work has laid a solid foundation of elucidating the synthesis and gene engineering of fatty acid of Cassia obtusifolia L.
出处
《生物技术》
CAS
CSCD
北大核心
2015年第2期103-108,共6页
Biotechnology
基金
国家重大新药创制专项项目子课题("葛黄有效组分防治2型糖尿病候选药物研究"
No.2013ZX09103002-014)资助
关键词
基因克隆
决明
MCAT
PCR
序列分析
gene cloning, Cassia obtasifolia L. , MCAT, PCR, sequence analysis
