摘要
以陕单609为材料,采用盆栽试验,设置中度、重度干旱胁迫2个处理,研究干旱胁迫对苗期玉米叶片光系统Ⅱ(PSⅡ)性能、干物质积累、保护酶活性及脯氨酸含量的影响.结果表明:随着干旱胁迫程度的增加,玉米干物质积累量、叶面积、株高下降显著;PSⅡ复合体的不稳定性加重(L-band>0),供体侧放氧复合体受到伤害(K-band>0),受体侧电子传递链受到抑制(Ψo显著下降),进而导致光系统Ⅱ整体性能下降(PIABS显著下降).在中度和重度干旱胁迫下,超氧化物歧化酶、过氧化氢酶和过氧化物酶活性,以及脯氨酸含量均显著增加,分别为对照的1.3、1.1、1.2、5.8倍和1.1、3.3、1.5、15.0倍.这表明干旱胁迫引起的玉米叶片光系统Ⅱ供受体侧的损伤是光系统Ⅱ性能下降的原因,导致玉米干物质生产下降,而保护酶和脯氨酸对玉米抵御干旱胁迫起到了积极的作用.
Maize cultivar Shandan 609 was used to determine the effects of drought stress on photosystemⅡ,dry matter accumulation,protective enzyme activity and proline content at seedling stage in pot experiment. Two drought treatments（ moderate drought and severe drought） were tested. The results showed that dry matter accumulation,leaf area and plant height of cultivar shaandan 609 decreased significantly with the increasing drought stress. The less grouped PSⅡunits（ L-band 〉0）,severely damaged OEC（ K-band〉0）,and the inhibited acceptor side（ Ψosignificantly decreased）resulted in the overall dropped performance of PSⅡ（ PIABS）. Under the condition of moderate and severe drought stress,the activities of superoxide,peroxidase,catalase and proline content increased significantly,which were 1. 3,1. 1,1. 2,5. 8 and 1. 1,3. 3,1. 5,15. 0 times of control（ CK）,respectively. Those results indicated that damage of PSⅡ acceptor and donor side under drought stress led to the decline of PSⅡ performance,which was likely to cause the decline of dry mater accumulation of maize cultivar Shandan 609,while protective enzymes and proline as protective substance in plant played a positive role in drought resistance.
出处
《应用生态学报》
CAS
CSCD
北大核心
2015年第5期1391-1396,共6页
Chinese Journal of Applied Ecology
基金
国家重点基础研究发展计划项目(2009CB118604)
陕西省科技计划农业攻关项目(2014K01-02-03)
西北农林科技大学创新专项(QN2011086)资助
