摘要
目的探讨泛素特异性蛋白酶9X(ubiquitin-specific protease,USP9X)在胰腺癌组织中的表达及其与预后的关系。方法应用免疫组织化学SP法检测USP9X在55例行根治性手术切除的原发性胰腺癌组织及其癌旁非肿瘤胰腺组织中的表达。结果胰腺癌组织中USP9X高表达率为58.2%(32/55),癌旁非肿瘤组织中为0(0/55)。USP9X的高表达率与患者的性别、年龄、肿瘤部位及肿瘤大小无关(P>0.05),而与肿瘤分化程度、有无淋巴结转移及TNM分期有关(P<0.05)。Cox比例风险分析结果显示,肿瘤分化程度、有无淋巴结转移及USP9X表达均是影响预后的独立危险因素。生存分析结果显示,USP9X高表达组患者的预后明显差于低表达组(P<0.05),且在TNM分期Ⅱ期、淋巴结阴性及肿瘤分化程度为中分化患者中也得到相同结果(P<0.05)。结论 USP9X与胰腺癌的发生、发展及预后密切相关。
Objective To investigate the expression of ubiquitin-specific protease 9X （USP9X） in pancreatic cancer, and to evaluate the correlation of USP9X with the survival of patients with pancreatic cancer. Method The expression of USP9X was detected in 55 pieces of surgically resected primary pancreatic cancer tissues and adjacent nontumorous pancreatic tissues by streptavidin-perosidase immunohistochemical method. Results The rate of USP9X high expression in the 55 pieces of the primary pancreatic cancer tissues was 58.2% （32/55）, which in the adjacent nontumorous pancreatic tissues was zero. The expression of USP9X was not correlated with the gender, age, tumor position, or tumor size （P〉0.05）, while which was significantly correlated with the differentiation degree, lymph node metastasis, or TNM stage （P〈0.05）. By using Cox proportional hazard model, the multivariable analysis revealed that the differentiation degree, lymph node metastasis, and USP9X expression were the independent risk factors. Survival of the patient with USP9X high expression was significantly shorter than that with USP9X low expression （P〈0.05）, and there was the same result in the patients with stage Ⅱ, with lymph node negative, or intermediate differentiation degree （P〈0.05）. Conclusion The results indicate that USP9X might play an important role in the pathogenesis and prognosis of pancreatic cancer.
出处
《中国普外基础与临床杂志》
CAS
2015年第5期601-604,共4页
Chinese Journal of Bases and Clinics In General Surgery
关键词
胰腺癌
泛素特异性蛋白酶9X
免疫组织化学方法
预后
Pancreatic cancer
Ubiquitin-specific protease 9X
Immunohistochemical method
Prognosis
