摘要
目的观察重组人白细胞抑制因子和水蛭肽嵌合蛋白(TNHH)对大鼠局灶性脑缺血的保护作用。方法本实验采用线栓法建立大鼠大脑中动脉阻塞(middle cerebral artery occlusion,MCAO)的局灶性脑缺血模型,将大鼠随机分为5组:1空白对照组,2假手术组,3脑缺血损伤模型组,4溶媒对照组和5人白细胞抑制因子和水蛭肽嵌合蛋白(6.75 mg·kg-1)组。持续性脑缺血12 h后进行神经行为学评分,处死动物后用2,3,5-氯化三苯基四氮唑(TTC)染色法测定脑梗死面积,并对脑组织切片进行HE染色检测脑组织病理损伤情况,酶联免疫分析(ELISA)和放射免疫法分别测定血清中炎症因子肿瘤坏死因子(TNF)-α和白细胞介素(IL)-1β水平。结果与缺血损伤模型组相比,人白细胞抑制因子和水蛭肽嵌合蛋白可以明显改善大鼠脑缺血损伤后的神经行为,减少脑梗死面积,减轻脑水肿程度和脑组织病理损伤,显著降低血清中肿瘤坏死因子-α和白细胞介素-1β水平。结论人白细胞抑制因子和水蛭肽嵌合蛋白具有明显的抗炎、抗水肿作用,可以有效改善大鼠局灶性脑缺血的损伤。
OBJECTIVE To investigate the protective effects of human recombinant neutrophil inhibitory factor and hirulog hybrid（ TNHH） on focal cerebral ischemia in rats. METHODS Middle cerebral artery occlusion model was prepared in rats to imitate the focal cerebral ischemia. Rats were divided into five groups： 1blank,2sham,3focal cerebral ischemia,4ischemia ＋ vehicle and5 ischemia ＋ TNHH（ 6. 75 mg·kg- 1）. After persistent ischemia for 12 h,neurologic deficit score was assayed,and then scarified the animals to measure the brain infraction area by TTC staining,the brain pathological damage by HE staining,and the levels of inflammatory factor TNF-α and IL-1β in serum respectively by using ELISA and radio-immunity assay. RESULTS TNHH could significantly improve the neurological outcome,decrease the infraction area,alleviate brain edema and pathological injury and reduce the levels of TNF-α and IL-1β in rats suffered persistent focal cerebral ischemia for 12 h. CONCLUSION TNHH has significant anti-inflammatory and anti-edema effects,which could effectively alleviate focal cerebral ischemia injury in rats.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2015年第10期863-867,共5页
Chinese Pharmaceutical Journal
关键词
人白细胞抑制因子和水蛭肽嵌合蛋白(TNHH)
局灶性脑缺血
抗炎作用
脑水肿
human recombinant neutrophil inhibitory factor and hirulog hybrid（TNHH）
focal cerebral ischemia
anti-inflammatory effect
brain edema
