摘要
目的：探讨 CD34、CD117、平滑肌肌动蛋白（SMA）、S-100蛋白在胃肠道间质瘤中的表达及与临床病理特征的关系。方法收集60例手术切除的胃肠道间质瘤标本，所有病例均经术后病理学确诊，免疫组化法检测 CD34、CD117、SMA、S-100蛋白在胃肠道间质瘤中的表达情况，分析其阳性表达与临床病理特征的关系。结果CD34阳性信号定位在细胞浆，CD117阳性信号主要定位于细胞浆，少数位于细胞膜，在细胞中呈核黄色颗粒弥漫性分布，SMA 和 S-100蛋白在细胞浆的局灶性或散点状阳性表达，CD34、CD117、SMA、S-100蛋白在胃肠道间质瘤组织中阳性表达率分别为88．33％（53／60）、95．00％（57／60），43．33％（26／60）和6．67％（4／60）；CD34阳性表达率与部位相关（χ2＝9．093，P ＜0．05），S-100蛋白阳性表达率与组织学类型相关（χ2＝10．447，P ＜0．05）。结论CD34、CD117在胃肠道间质瘤组织中阳性表达率很高，联合 CD34、CD117检测可以提高胃肠道间质瘤诊断准确率。
Objective To explore the expression and relationship with the clinical pathological features of CD34,CD117,smooth muscle actin（SMA）,S -100 protein in gastrointestinal stromal tumor.Methods 60 cases of resection of gastrointestinal stromal tumor specimens were collected,all cases were confirmed by postoperative patholo-gy,explored the expression of CD34,CD117,SMA,S -100 protein expression in gastrointestinal stromal tumor with immunohistochemical method,analyzed the relationship with the clinical pathological features of CD34,CD117,SMA, S -100 protein.Results CD34 positive signal located in the cytoplasm,CD117 positive signal mainly located in cyto-plasm,a few located in the cell membrane,nuclear yellow particles diffuse distribution in cells,SMA and S -100 pro-tein located in the cytoplasm of focal or scattered point positive expression of CD34,CD117,SMA,S -100 protein pos-itive expression rate in gastrointestinal stromal tumor tissue were 88.33% （53 /60）,95.00% （57 /60）,43.33%（26 /60）and 6.67% （4 /60）;CD34 positive expression rate was related to area （χ2 =9.093,P 〈0.05）,S -100 protein positive expression rate related to the histological type （χ2 =10.447,P 〈0.05）.Conclusion CD34,CD117 in gastrointestinal stromal tumor tissue in the positive expression rate is very high,detection jointed with CD34,CD117 can improve the gastrointestinal stromal tumor diagnosis accuracy.
出处
《中国基层医药》
CAS
2015年第10期1499-1502,I0002,共5页
Chinese Journal of Primary Medicine and Pharmacy
