摘要
目的:通过对白裤瑶族线粒体DNA D-Loop HVRⅠ区序列碱基的测定,了解其遗传多态性。方法:应用PCR扩增产物及直接测序法,对31名白裤瑶无血缘关系健康个体mt DNA HVRⅠ区进序列检测及分析。结果:与Anderson相应序列比对,白裤瑶群体mt DNA HVRⅠ区发现了88处突变,界定了3种单倍型,基因差异度分别为1.00,偶合概率为0.25。结论:广西白裤瑶线粒体DNA HVRⅠ区多态性有一定的本民族特点。
Objective： to study the genetic polymorphism of mitochondrial DNA in Baiku Yao nationality.Methods：The HVR I region of mtDNA in 31 Baiku Yao from Guangxi were detected by PCR and examined by DNA sequencing. Results： Compared to Cambridge reference sequence, 88 polymorphic sites of HVR I were found in Balku yao mtDNA sequence, 4 haplotypes were defined.The genetic diversity was calculated as 1.00, and the genetic identity was 0.25. Conclusion： The national characteristics of Balku yao show unique features compared with those of others.
出处
《科技视界》
2015年第16期46-46,11,共2页
Science & Technology Vision
基金
2011年新世纪广西高等教育教改工程项目(2011JGB084)
桂林医学院2009年度青年教师科研启动基金项目(院科字(2008)18号)
2013年度桂林医学院教学研究与改革委托课题(院教发字[2013]23号
