摘要
目的 探讨重组人干扰素α1b（rhIFN-α1b）不同给药方式及剂量对呼吸道合胞病毒（RSV）感染小鼠的抗病毒作用及对酪氨酸蛋白激酶/信号传导与转录活化因子（JAK/STAT）信号通路的影响.方法 将48只BALB/c小鼠按随机数字表法随机分为6组：阴性对照组,RSV感染模型组,rhIFN-α1b 12.5 μg、25.0μg、50.0 μ,g雾化吸入组和12.5μg/kg腹腔注射组.连续5d药物干预治疗,第6天无菌剖取小鼠左肺组织,HE染色观察各组小鼠肺组织病理学改变;免疫荧光染色观察STAT1和STAT2蛋白表达情况.结果RSV感染模型组肺组织炎性损伤明显,rhIFN-α1b治疗后炎性损伤均有不同程度恢复,其中rhIFN-α1b 50.0 μg雾化吸入组炎性损伤程度最轻,各组间比较差异均有统计学意义（P均＜0.05）.RSV感染模型组STAT1和STAT2蛋白表达减弱,rhIFN-α1b治疗后不同程度提高受RSV抑制的STAT1、STAT2蛋白表达,rhIFN-Mb 50.0 μg雾化吸入组STAT1、STAT2蛋白表达增强最显著,各组间比较差异均有统计学意义（P均＜0.05）.结论rhIFN-α1b对RSV有抗病毒作用,同时可提高受RSV感染小鼠抑制的JAK/STAT信号通路的活性,雾化吸入治疗效果优于腹腔注射,且在一定范围内雾化吸入的疗效与剂量呈正比.
Objective To investigate the impact of different ways and doses of recombinant human interferon αlb（rhIFN- otlb） on antiviral effect on respiratory syncytial virus（RSV） -effected mice and signal pathway of JAIL/ STAT. Methods Forty - eight mice were divided randomly into 6 groups, negative control group, RSV - infection model group, 12.5,25.0,50.0 αg rhIFN -αl b atomization inhalation of intervention group, and 12.5 αg rhIFN- αl b injection of intervention group. After continuous drug therapy for 5 days, the left lung tissues of mice were aseptically dissected in the sixth day. Then it were observed lung tissue pathology changes by optical microscope, and expression of STAT1 and STAT2 protein with laser scanning confocal microscopy. Results It was observed that RSV - infection model mice＇s lungs had significant inflammatory injury under light microscope. After treatments of rhIFN - αlb, it showed that the mice lung tissue had recovery of inflammation on different degrees. The group of inhaled rhIFN - αlb 50.0 μg was damaged on lightest degree. There were statistically significant differences between each group （ all P 〈 0. 05 ）. The expression of STAT1 and STAT2 protein in RSV - infection model mice＇s lungs decreased remarkably under flnorescene microscopy. Treatments of rhlFN -αlb increased the expression of STAT1 and STAT2 inhibited by RSV - infection. The expression of STAT1 and STAT2 protein of the inhaled rhIFN - αlb 50.0 p,g group increased significant- ly. The differences between groups were statistically significant （ all P 〈 0.05 ）. Conclusions rhIFN - αlb b played an important role in anti - RSV effects, simultaneously, it could improve the activity of JAK/STAT signal pathway inhibi- ted by RSV - infection. The treatment effect of rhIFN - αlb deliveried by atomization inhalation was better than that of intraperitoneal injection. And curative effect is proportional to the atomization inhalation dose within a certain range.
出处
《中华实用儿科临床杂志》
CAS
CSCD
北大核心
2015年第10期772-775,共4页
Chinese Journal of Applied Clinical Pediatrics
基金
国家“十一五”科技支撑项目子课题（077BA107A12）
关键词
呼吸道合胞病毒
重组人干扰素αlb
肺组织病理学
信号传导与转录活化因子
Respiratory syncytial virus
Recombinant-- human interferon （xlb
Lung tissue pathology
Signaltransducer and activator of transcription
