摘要
目的:比较宫颈上皮内瘤变(CIN)组织标本中人乳头瘤病毒(HPV)感染的基因型分布情况及其临床意义。方法:采用聚合酶链式反应和基因芯片检测技术对20例宫颈正常组织、145例宫颈CINⅠ级、77例宫颈CINⅡ级和94例宫颈CINⅢ级组织标本进行23种HPV基因分型检测,并对受检者进行相关资料分析。结果:20例宫颈正常组织检出HPV感染者2例,感染率为10.00%;145例宫颈CINⅠ组织检出HPV感染者61例,感染率为42.07%;77例宫颈CINⅡ组织检出HPV感染者67例,感染率为87.01%;94例宫颈CINⅢ组织检出HPV感染者85例,总的HPV感染率为90.43%。结论:聚合酶链式反应与基因芯片检测技术可应用于宫颈上皮内瘤变组织标本,1次可检测23种HPV基因型别,对我国女性宫颈病变HPV感染基因型分布的研究及宫颈癌的防治及其疫苗的研发具有十分重要的意义。
Objective： To compare the distribution of genotypes of human papillomavirus （HPV） infection in cervical intraepitheli- al neoplasia （CIN） and the clinical significance. Methods： PCR and gene chip technology were used to detect 23 kinds of HPV genotypes in 20 cases with normal cervical tissue, 145 cases with CIN I , 77 cases with CIN Ⅱ and 94 cases with CIN Ⅲ. The related data of the cases was analyzed. Results： Among 20 cases with normal cervical tissue, ：2 cases were diagnosed as HPV infection, the infection rate of HPV was 10. 00% ; among 145 cases with CIN I , 61 cases were diagnosed as HPV infection, the infection rate of HPV was 42. 07% ; among 77 cases with CIN I , 67 cases were diagnosed as HPV infection, the infection rate of HPV was 87.01% ; among 94 cases with CINⅢ, 85 cases were diagnosed as HPV infection, the infection rate of HPV was 90. 43%. Conclusion： PCR and gene chip technology can be used for CIN specimens, 23 kinds of HPV genotypes can be detected at the same time, which are important in studying the distribution of genotypes of HPV infection in CIN cases, prevention and treatment of cervical cancer, and development of HPV vaccine.
出处
《中国妇幼保健》
CAS
2015年第15期2333-2336,共4页
Maternal and Child Health Care of China
基金
南京市卫生局中医专项支助项目〔2009-92〕
关键词
宫颈上皮内瘤变
组织
人乳头瘤病毒
基因分型
Cervical intraepithelial neoplasia
Tissue
Human papillomavirus
Genotyping
