摘要
目的:基于Tau蛋白磷酸化探讨电针百会对APP/PS1转基因痴呆模型小鼠学习记忆能力的影响及其可能机制。方法:30只4月龄雌性APP/PS1转基因小鼠随机分为模型组、百会组和非穴组,10只同窝阴性野生小鼠为野生组;百会组电针百会穴,非穴组电针非穴,干预28d。采用Morris水迷宫实验观察小鼠学习记忆能力;尼氏染色观察小鼠海马神经元形态结构变化;蛋白免疫印记杂交技术(Western blot)检测小鼠海马组织在Ser-396位点上Tau蛋白磷酸化水平。结果:与模型组相比,百会组可改善小鼠的学习记忆能力(P<0.05),减轻海马神经元形态结构的损伤,降低海马组织中Ser-396位点上Tau蛋白磷酸化水平(P<0.05),非穴组与模型组相比无显著性差异(P>0.05)。结论:电针百会能够改善APP/PS1转基因痴呆模型小鼠的学习记忆能力,其机制可能与抑制Tau蛋白磷酸化有关。
Objective： To explore the effects of electroacupuncture at Baihui （DU20） on learning and memory ability and its possible mechanism through the phosphorylated Tan in APP/PS1 transgenic mice. Method： Thirty female APP/PS1 transgenic dementia model mice were randomly divided into model group, DU20 group and non-acupoint group, 10 wild type mice consisted wild group. The DU20 group received electroacupuncture at Baihui （DU20） and the non-acupoint group received electroacupuncture at non-acupoint for 28 days. Learning and memory ability was tested with Morris water maze. The morphology and structure of hippo- campus neurons were observe by Nissl＇s staining. The level of Tau phosphorylation at Ser-396 point was detect- ed by Western blot. Result： Compared with the model group, electroacupuncture at DU20 could ameliorated learning and memory ability of APP/PSI mice （P〈0.05）, decreased the injury of neurons in morphology and structure and the level of Tan phosphorylation at Ser-396 in hippocampus （P〈0.05）, and there was no significant difference between model group and non-acupoint group （P 〉 0.05）. Conclusion： Electroacupuncture at DU20 could ameliorate learning and memory ability in APP/PS1 transgenic dementia model mice, the mechanism may be related to the Tan pbosphorylation in hippocampus.
出处
《中国康复医学杂志》
CAS
CSCD
北大核心
2015年第5期432-436,共5页
Chinese Journal of Rehabilitation Medicine
基金
福建省康复技术协同创新中心资助项目(X2012004-协同)
关键词
电针
阿尔茨海默病
学习记忆
TAU
electroacupuncture
Alzheimer＇s disease
learning and memory
Tau
