摘要
目的 建立工作场所空气中 N-异丙基苯胺测定的高效液相色谱方法.方法 采用硅胶管吸附空气中的N-异丙基苯胺,乙腈解吸,高效液相色谱-紫外检测器测定.结果 该方法在0.0～100.0μg/ml范围内呈线性关系,回归方程为y=22 863x＋10 665,相关系数r=0.999 9;检出限为0.005 μg/ml,最低检出浓度为1.7×10-3mg/m3（采样体积以3.0L计）,平均加标回收率为96.2％～101.3％,精密度批内为2.31％～2.99％,批问为3.21％～4.55％,平均解吸效率为97.6％,穿透容量大于8.12 mg,采样效率为100％,样品在室温下至少可保存7 d. 结论 该方法各项指标均符合GBZ/T 210.4-2008《工作场所空气中化学物质测定方法》的要求,适用于工作场所空气中N-异丙基苯胺的检测.
Objective To establish the method of high-performance liquid chromatography （HPLC） for the determination of N-isopropylaniline in workplace air.Methods N-isopropylaniline in the air was collected by silicone tube.and was then dissolved by acetonitrile and determined by HPLC-UV detector.Results There was a linear relationship within the range of 0.0～100.0 μg/ml with the method,and the regression equation was y=22 863x＋ 10 665 （r=0.999 9）;the detection limit was 0.005 μg/ml,and the minimum detectable concentration was 1.7×10-3 mg/m3 （3.0 L sampling volume）;the average recoveries of standard addition were 96.2%～101.3%.The within-run precision was 2.31%～2.99%,and the between-run precision was 3.21%～4.55%.The average desorption efficiency was 97.6%,the breakthrough volume was more than 8.12 mg,the sampling efficiency was 100%,and the samples could be stored for at least 7 days at room temperature.Conclusion The indicators of the method all meet the requirements of GBZ/T 210.4-2008 （Determination methods of air chemicals in workplace）,and can be used for the determination of N-isopropylaniline in workplace air.
出处
《中华劳动卫生职业病杂志》
CAS
CSCD
2015年第4期294-296,共3页
Chinese Journal of Industrial Hygiene and Occupational Diseases
关键词
N-异丙基苯胺
工作场所
色谱法
高效液相
N-isopropylaniline
Workplace
Chromatography,high performance liquid
