摘要
目的探讨瑞舒伐他汀对大鼠脑缺血再灌注后白细胞介素-1β(IL-1β)和白细胞介素-12(IL-12)的影响。方法大鼠随机分为3组,假手术组、脑缺血再灌注组(模型组)和瑞舒伐他汀组预处理组(实验组)。实验组在模型制作前用瑞舒伐他汀5 mg·kg-1·d-1连续灌胃10 d,1次/天。缺血1 h再灌注12 h后,进行神经功能评分,断头取脑,用酶联免疫吸附法和聚合酶链反应法检测大鼠的脑组织中IL-1β和IL-12的表达。结果与模型组相比,实验组大鼠神经功能评分显著降低,IL-1β和IL-12表达显著下调(均P<0.05)。结论瑞舒伐他汀促进大鼠脑缺血再灌注损伤后神经功能的恢复,可能与抑制炎症因子的表达有关。
Objective To observe the effects of rosuvastatin on the expression of interleukin-1β（ IL -1β） and interleukin -12 （ IL-12 ） in rats with focal cerebral ischemia-reperfusion.Methods Sprague-Dawley rats were randomly assigned into three groups： Sham operation group, focal cerebral ischemia-reperfusion group（model group） and rosu-vastatin preconditioning group （ test group） .Before making a model, the rats in the test group were lavaged with rosuvastatin 5 mg· kg-1 · d-1 , once a day for consecutive days.The middle cerebral artery occlusion reperfu-sion model was made by the suture method （ ischemia for 1 hour, and reperfusion for 12 hours）.The level of IL-1βand IL-12 expression were measured by enzyme -linked immunosorbent assay （ ELISA ） and polymerase chain reaction （ PCR ）.Results Compared with model group, the expression of IL-1βand IL-12 in the rats of test group were downregulated （ P〈0.05 ）.Conclusion Rosuvastatin can relieve the brain damage induced by focal cerebral ischemia -reperfusion, whose mechanisms seemed related to inhibiting the expression of IL-1βand IL-12.
出处
《中国临床药理学杂志》
CAS
CSCD
北大核心
2015年第9期744-746,共3页
The Chinese Journal of Clinical Pharmacology
基金
福建省教育厅B类课题基金资助项目(JB13303)
泉州市科技计划基金资助项目(2012Z78)
