摘要
目的：评价比较不同配比壳聚糖/聚磷酸钙（Chitosan/Calcium polyphosphate,CS/CPP）复合支架的细胞相容性和组织相容性,探讨其应用于组织工程半月板支架材料的可行性。方法：采用扫描电镜检测半月板细胞在支架材料上的生长情况。采用MTT法检测不同配比CS/CPP支架材料浸提液对第3代半月板细胞的毒性,通过肌袋内植入试验评价支架材料的体内组织相容性。结果：扫描电镜下可见：不同比例CS/CPP复合支架材料呈三维多孔的结构。复合第3代半月板细胞体外培养7 d,细胞在支架材料上均匀分布,紧贴支架铺展伸长,生长良好。MTT法显示不同浓度支架浸提液与对照DF培养液吸光度值比较,差异无统计学意义（P〉0.05）,支架无细胞毒性。肌袋内植入试验评价：肌袋内植入实验显示动物术后生存良好,各植入部位均未见组织坏死、积液及化脓感染。术后1周植入体周围有较多中性粒细胞浸润;术后2~8周植入体周围中性粒细胞浸润逐渐减少,出现淋巴细胞浸润,淋巴细胞多于中性粒细胞,纤维囊壁厚薄不均;3个月后植入体周围未见炎症细胞浸润,主要为胶原纤维和成纤维细胞。随植入时间的延长,炎症细胞浸润逐渐减少,浸润范围逐渐缩小,包膜先逐渐变厚再变薄,植入材料内部逐渐有组织长入,并且逐渐降解。结论：CS/CPP具有良好的细胞相容性和组织相容性,有望成为半月板组织工程的支架材料。
Objective To investigate the biocompatibility of chitosan（CS）/calcium polyphosphate（CPP）scaffold with different proportions of CS and CPP. Methods The growth of fibrochondrocytes on CS/CPP scaffold was observed by scanning electron microscope. The cytotoxicity of different proportions of CS/CPP leaching liquors was assessed by MTT. Results There was no cell toxicity composite of CS/CPP scaffolds with different proportions. Mass neutrophils infiltrating existed in implanted muscle one week after the operation and decreased within 2~8 weeks after the operation, while the lymph cells infiltrating gradually emerged. The neutrophils and lymph cells disappeared and collagenous fibers and fibroblasts appeared 3 months after the implantation. The tissue gradually grew into the CS/CPP scaffold and the CS/CPP degraded gradually with extension of implantation duration. Conclusion The CS/CPP scaffold with different proportions has perfect cytocompatibility and histocompatibility.
出处
《中国运动医学杂志》
CAS
北大核心
2015年第2期117-122,共6页
Chinese Journal of Sports Medicine
基金
国家自然科学基金(81301560)资助
关键词
壳聚糖/聚磷酸钙
细胞相容性
组织相容性
半月板细胞
chitosan/calcium polyphosphate
cellular compatibility
biocompatibility
meniscal cells
