摘要
通过测定斑点气单胞菌单药和双药抗生素最低抑菌浓度(MIC)、防突变浓度(MPC)和耐药突变选择窗(MSW),探索合理使用抗菌药物,防止细菌耐药性产生的用药方案。利用药物敏感性实验测定斑点气单胞菌对10种抗生素的抗性,筛选出6种不同机制的敏感性抗菌药物,应用琼脂平板稀释法对斑点气单胞菌测定单药以及双药的最低抑菌浓度(MIC)和防突变浓度(MPC),计算选择指数(SI)和联合指数(CI),测定联合用药对耐药突变选择窗(MSW)的影响。结果显示:6种药物氯霉素、土霉素、利福平、红霉素、盐酸四环素、头孢霉素的MIC分别为0.512、0.416、6.272、2.048、0.386和0.512μg/m L;MPC分别为524.288、1 277.952、802.816、1 024.000、1 185.792和10.880μg/m L;SI值分别为1 024.000、3 072.000、128.000、500.000、3 072.000和21.250。土霉素与红霉素、盐酸四环素与红霉素、盐酸四环素与头孢霉素、土霉素与头孢霉素、盐酸四环素与土霉素、氯霉素与土霉素、氯霉素与盐酸四环素、利福平与氯霉素、利福平与头孢霉素、利福平与盐酸四环素、利福平与土霉素、红霉素与头孢霉素双药联合后的联合指数分别为1.000、1.000、1.000、1.000、0.834、1.500、1.500、0.834、2.000、2.000、1.000和2.000。双药联合明显降低了药物SI值,有效缩小了斑点气单胞菌耐药突变选择窗,其中利福平与盐酸四环素双药联合后SI值由128.000,3 072.000均降低至1.000,完全关闭了耐药突变选择窗,有效抑制斑点气单胞菌的耐药生长。
The purpose of this study is to explore the rational use of antimicrobial drugs, and prevent bacterial resistance to antibiotics through determination minimal inhibitory concentration （MIC）, mutant prevention concentration（MPC） and mutant selection window （MSW） of monotherapy and double medicine antibiotics on Aeromonas punctata. Resistances of 10 antibiotics were tested via drugs sensitivity assays, 6 sensitive antimicrobial drugs with different mechanisms were screened and their MIC, MPC, selection index （SI） and MSW to Aeromonas punctata were determined respectively via single-drug and double-drug＇ s combination by agar plate dilution method. The MIC of chloramphenicol, oxytetracycline, rifampicin, erythromycin, tetracycline hydrochloride, cephalosporins were 0. 512,0.416,6. 272,2. 048,0. 386,0.512 ixg/mL, respectively. The MPC of 6 antibiotics were 524. 288,1 277. 952,802.816,1 024. 000,1 185. 792, 10. 880 ixg/mL. The SI were 1 024. 000, 3 072. 000, 128. 000,500. 000, 3 072. 000, 21. 250. The combination index of oxytetracycline and erythromycin, tetracycline hydrochloride and erythromycin, tetracycline hydrochloride and cephalosporins, oxytetracycline and cephalosporins, tetracycline hydrochloride and oxytetracycline, chloramphenicol and oxytetracycline, chloramphenicol and tetracycline hydrochloride, rifampicin and chloramphenicol, rifampicin and cephalosporins, rifampicin and tetracycline hydrochloride, rifampicin and oxytetracycline, erythromycin and cephalosporins were 1. 000,1. 000,1. 000,1. 000,0. 834, 1. 500,1. 500,0. 834,2. 000,2. 000,1. 000,2. 000, respectively. It was confirmed that the mutant selection window of A. punctata was narrowed effectively via double drug combination. The Sis declined from 128. 000 and 3 072. 000 to 1. 000 and 1. 000 after combination of rifampicin and tetracycline, so this demonstrated that combination of rifampicin and tetracycline closed the resistant mutant selection window, and inhibited effectively the resistant growth of A. punctata.
出处
《水产学报》
CAS
CSCD
北大核心
2015年第4期566-572,共7页
Journal of Fisheries of China
基金
国家自然科学基金(30871648)
广东省教育部产学研专项(2011B090400416)
中山市科技计划专项(20123A317)
电子科技大学中山学院科研团队培育专项(412YT02)
