摘要
目的观察甜菜碱对复合致病因素诱导肝纤维化大鼠的保护作用。方法复合致病因素法诱导制备肝纤维化大鼠模型,观察灌服甜菜碱对肝纤维化大鼠血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、内毒素(ET)、同型半胱氨酸(Hcy)、透明质酸(HA)、层粘连蛋白(LN)、IV胶原蛋白(IV-C)、Ⅲ型前胶原蛋白(PC-Ⅲ)及肝组织肿瘤坏死因子α(TNF-α)、一氧化氮(NO)、过氧亚硝酸盐阴离子(ONOO-)、丙二醛(MDA)的变化,并采用HE和VG染色分别观察肝损伤和肝纤维化情况。结果大鼠肝纤维化模型复制成功。给与三个剂量的甜菜碱后,肝组织病理损伤改善。反映大鼠肝功能(ALT、AST)及肝纤维化指标(HA、LN、IV-C)的水平不同程度降低。血清内毒素、肝组织NO、MDA水平亦明显降低。同时Hcy及ONOO-在中、高剂量组明显降低,而TNF-α的降低在高剂量组中也具有明显的统计学差异。结论甜菜碱可抑制复合因素诱导的大鼠肝纤维化,其作用可能是通过降低大鼠体内高同型半胱氨酸、调节炎性细胞因子和氧化应激实现的。
Objective To explore the protective effects of betaine on liver fibrosis in rats and the possible mechanisms involved. Methods SD rats were randomly divided into 5 groups： control, liver fibrosis model, low-dose betaine（250 mg/kg）, medium-dose betaine（500mg/kg） and high-dose betaine（1000 mg/kg）. The rats in the three betaine groups were treated with betaine by injection. In the 4th week, blood and liver tissues were collected to assay serum endotoxin, markers for liver fibrosis, homocysteine（Hcy） and hepatic tumor necrosis factor α（TNF-α）, NO, peroxynitrite（ONOO-）, malondialdehyde（MDA）. The liver injury and hepatic fibrosis were observed with the staining of HE and VG, respectively. Results The rats in the liver fibrosis group displayed higher serum levels of endotoxin, markers for liver fibrosis and Hcy. After betaine injection, the rats in the three betaine groups had lower serum levels of endotoxin and markers for liver fibrosis, and lower hepatic levels of NO, MDA compared with hepatic fibrosis group. Conclusion Betaine can play a role in inhibiting the development of liver fibrosis in rats induced by multiple pathogenic factors. The protective effects were associated with lowering Hcy, regulating inflammatory cytokines and scavenging free radicals.
出处
《营养学报》
CAS
CSCD
北大核心
2015年第2期164-168,共5页
Acta Nutrimenta Sinica
基金
国家自然科学基金(No.81070339)
山西省国际科技合作计划项目(No.2010081068)
山西省回国留学人员科研资助项目(No.211-091)
山西医科大学细胞生理学省部共建教育部重点实验室主任基金资助项目(No.2010-09)
关键词
甜菜碱
肝纤维化
内毒素
betaine
hepatic fibrosis
endotoxin
