摘要
生物胺是低分子量有机碱,存在于蛋白质丰富的食品尤其是发酵食品中。测定食品中的生物胺方法较多,每种方法都有自己优点和缺点。主要介绍了咖啡中生物胺测定的三种方法:反相高效液相色谱法、离子色谱法和薄层色谱法。重点介绍了三种方法的特点及其在咖啡中的生物胺测定中的应用,并总结了在各种类咖啡生豆、烘焙咖啡中的生物胺含量检测方法与技术,给出了咖啡中的生物胺含量检测结果。
Biogenic amines are low molecular weight organic bases, present in protein-rich foods, especially in fermented products. Eating too much biogenic amine can cause allergic reactions in human body, even can endanger the life in acute cases. There are many methods for determining the biogenic amines in foods and each method has its own advantages and disadvantages. Mainly describe three methods of biogenic amines content determination in coffee： reverse phase high performance liquid chromatography, ion-pair reverse phase high performance liquid chromatography and thin layer chromatography. Highlight the characteristics of these methods and their application in biogenic amines determination in coffee. Summarize the methods and technologies for detecting biogenic amines contents in both green and roasted coffee beans, and the progress in recent technologies for determining the impact ofbiogenic amines contents.
出处
《食品工业》
北大核心
2015年第5期236-241,共6页
The Food Industry
基金
公益性行业(农业)科研专项资金项目(201003063-07)
