摘要
目的分析吸烟、喝酒、桑拿生活习惯对精子DNA碎片化的影响，寻找不育症患者的不育影响因素。方法在113例门诊患者中，77例男性不育症患者分为3组：A组为有吸烟／喝酒习惯31人，B组为有桑拿习惯21人，C组为其他不育症25人。另外D组为生育能力正常36人。应用精子染色质结构分析（SCSA）检测以上各组的精子DNA碎片化指数（DFI），分别比较各组间的差异。结果精子DNA碎片化指数（DFI）：A组为（29．03±11．41）％、B组为（28．12±9．56）％、C组为（24．08±6．27）％、D组为（14．87±3．54）％。A、B组分别高于C组，A、B组问比较差异无统计意义（Jp〉0．05），A、B组分别与c组比较有差异（P〈0．05）；A、B、C组分别与D组比较差异有显著性意义（P〈0．01）。结论男性不育症患者精子DNA碎片化指数（DFI）与正常生育人群右昂菩忡善导．不良生活习惯会加重精子DNA碎片化指数（DFI），可能是引起男性不育的因素。
Objective To investigate the effects of cigarette, alcohol and taking sauna on DNA fragmentation index （DFI） in infertile males, and find out impact factors related to infertility. Methods Total of 113 male patients including 77 infertile males and 36 with normal reproductive ability were recruited in the study. All patients were classified into Group A（31 infertile males with a habit of smoking/taking alcohol）, Group B（ 21 infertile males with a habit of taking sauna）, Group C（25 infertile males with other relative pathogenesis） and Group D（36 males with normal reproductive ability as control）. Semen samples were collected from each patients and their DFI were detected by a sperm chromatin structure assay （SCSA）. Results DNA fragmentation index in Group A, Group B, Group C and GroupD was 29.03±1.41%, 28.12±9.56%, 24.08±6.27%, and 14.87±3.54%, respectively. No significant difference in DFI was found between Group A and B（P〉0.05）. DFI in Group A and Group B were significantly higher than that in Group C （P〈0.05）. DFI in Group A, B and C was significantly different from that in Group D, respectively （P〈0.01）. Conclusion DFI is higher in infertile males than that in males with normal reproductive ability. And unhealthy lifestyle may affect DFI of their sperm, and further result in infertility.
出处
《中国男科学杂志》
CAS
CSCD
2015年第2期39-42,共4页
Chinese Journal of Andrology
关键词
吸烟
饮酒
蒸气浴
DNA碎片裂
smoking
Alcohol Drinking
Sauna
DNA fragmentation
