摘要
本研究利用波谱学方法对金龟子绿僵菌（Metarhizium anisopliae）在添加了熊果酸溶液的培养基中生物转化所得的产物进行纯化及结构鉴定，并对其生物转化条件进行正交优化。研究结果表明，金龟子绿僵菌在添加了熊果酸溶液的培养基中生物转化所得主要产物为烟曲霉酸，其产量比未添加熊果酸溶液时明显增多，最佳生物转化条件为：培养基pH为6．5，摇床转速为160rpm，反应温度为28℃。
In this study, the biotransformation products of ursolic acid by Metarhizium anisopliae were isolated and purified. The chemical structure of the isolated product was identified by spectral analysis. The biotransformation conditions were optimized using single factor and orthogonal experiments. The results showed that the main product of the biotransformation process was helvolic acid. The yield of helvolic acid was obviously increased using ursolic acid as the reaction medium. The optimal biotransformation conditions were obtained as follow：28 ℃ as reaction temperature, pH 6.5 and 160 rpm as the rotation speed of the incubator.
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2014年第A01期87-92,共6页
Natural Product Research and Development
关键词
金龟子绿僵菌
熊果酸
结构鉴定
烟曲霉酸
Metarhizium anisopliae
ursolic acid
spectral analysis
helvolic acid
