摘要
以假酸浆籽为原料,采用水提法、超声波提取法、微波提取法提取假酸浆多糖,经对各提取过程比较分析可知水提法为最优提取方法,提取条件为料液比1∶55、温度80℃、时间3 h,提取率为6.18%.将水提法得到的假酸浆多糖浓缩液通过醇沉、脱蛋白、脱色等工艺进行纯化,确定了最佳的提取纯化工艺.在最佳的条件下,醇沉后多糖的量可达80.5%,蛋白质脱除率可达70.8%,脱色率可达91.0%.
Nicandra polysaccharide was extracted from the seed of Nicandra physaloides （ L. ） Gaertn by the method of water extraction, ultrasonic extraction, microwave extraction, respectively. Through comparative analysis on the extraction process, water extraction achieving 6.18% extraction rate was determined to be the optimal method, in which the ratio of material to liquid was 1 ： 55, the extraction temperature was 80 ℃, and the extraction time was 3 h. The treatments of ethanol precipitation, deproteinization and decolorization were carried out for purifying to form the final polysaccharide. After the ethanol precipitation, the content of polysaccharide reached 80.5% , the protein removal rate reached 70.8% and the decolorization rate reached 91.0%.
出处
《哈尔滨商业大学学报（自然科学版）》
CAS
2015年第1期28-32,共5页
Journal of Harbin University of Commerce：Natural Sciences Edition
关键词
假酸浆多糖
提取
纯化
Nicandra polysaccharide
extraction
purification
