摘要
目的:探讨巴戟天与雌激素对骨质疏松大鼠破骨细胞RANK和CAII的表达影响。方法:选用4月龄SPF级健康成年雌性SD大鼠,建立去势大鼠骨质疏松模型,分离大鼠原代破骨细胞并分为3组:B组给予17β-雌二醇10-6mmol/L,C组给予1.0 mmol/L巴戟天,D组给予17β-雌二醇10-6mmol/L和1.0 mmol/L巴戟天进行培养,观察比较各组CAII、RANKmRNA表达等指标差异。结果:B、C和D组破骨细胞数量均较A组明显减少,而D组减少最明显(P<0.05);B、C、D组骨吸收陷窝面积相均低于A组(P<0.05),而D组骨吸收陷窝面积明显低于其他组(P<0.05);B、C、D组CAII、RANKmRNA基因表达水平明显低于A组(P<0.05),B组和C组RANKmRNA基因表达水平差异无统计学意义(P>0.05),而CAIImRNA基因表达水平差异有统计学意义(P<0.05),D组CAII、RANKmRNA基因表达水平最低(P<0.05)。结论:巴戟天联合雌激素能明显降低骨质疏松大鼠破骨细胞RANK和CAII的表达,起到抑制骨质疏松的作用。
Objective：To discuss the expression effect of Morinda Root and estrogen on Osteoporosis rat Osteoclast RANK and CAll. Method： Choose 4 months SPF health adult female SD mice, set up castrate rat Osteoporosis model, sepa- rate rat original Osteoclasts and divided into three groups：group B was given 17 [3 -estradiol 10-6 mmol/L, group C was given Morinda Root 1.0 mmol/L, group D was given 17 [3 - estradiol 10 -6mmol/L and Morinda Root 1.0 mmol/L to cul- tivate, observe and compare the difference of CAll and RANKmRNA expression of groups. Result：Osteoclast amount of group B ,C and D were less than A group obviously, while group D decrease was the most obvious（ P 〈0.05 ） ;group B, C and D bone resorption lacuna area were less than that of group A （P 〈 0.05 ）, while group D bone resorption lacuna area was less than that of other groups （ P 〈 O. 05 ） ;CAII and RANKmRNA gene expression level of group B, C and D were less than A group obviously（ P 〈 0.05 ） , RANKmRNA gene expression level of group B and C had no significant meaning（ P 〉 0.05 ） ,while CAIImRNA gene expression level had the significant meaning（P 〈0.05） ,CAll and RANKmRNA gene ex- pression level of group D was the lowest P 〈 0.05 ）. Conclusions ： Morinda Root and estrogen can reduce CAll and RANK gene expression of Osteoporosis rat Osteoclast obviously, having the effect of inhibiting osteoporosis.
出处
《中华中医药学刊》
CAS
北大核心
2015年第5期1224-1226,I0019,I0020,共5页
Chinese Archives of Traditional Chinese Medicine
