摘要
目的:建立白芍麸炒前后的高效液相(HPLC)指纹图谱。方法:分别选取白芍生品和制品各10批,以70%甲醇为提取溶剂,应用Agilent Eclipse XDB-C 18(4.6 mm×250 mm,5μm)柱,以0.1%磷酸溶液-乙腈为流动相进行梯度洗脱,流速l m L/min,柱温30℃,检测波长230 nm。结果:从白芍生品和炮制品中分别检测到17和18个共有色谱峰,并指认了4个色谱峰。研究发现特征图谱中主要色谱峰发生了改变。结论:从整体上显示白芍炮制前后指纹图谱的特征,为白芍饮片的质量和临床合理用药提供科学依据。
Objective ：to establish a high performance liquid of Radix Paeoniae Alba bran fried before and after the phase （HPLC） fingerprint. Method：select white peony root crude and products of each 10 group, with 70% methanol as extraction solvent, the application of AgilentEclipse XDB - C 18 （4.6 mm ×250 ram, 5 μm）, with 0.1% phosphoric acid solution column, acetonitrile as mobile phase and gradient elution, flow rate of L mL/min, column temperature of 30 DEG C, the detection wavelength of 230nm. Results： from the crude and processed products of Radix Paeoniae Alba were detected in 17 and 18 common peaks, and identify the 4 chromatographic peaks. The study found that changed the main chromatographic peaks in the fingerprint. Conclusion：display thecharacteristic fingerprint of Radix Paeoniae Alba before and after processing as a whole, and provide scientific basis for the quality of Radix Paeoniae Alba decoction pieces and clinical rational drug use.
出处
《中华中医药学刊》
CAS
北大核心
2015年第5期1056-1057,I0002,共3页
Chinese Archives of Traditional Chinese Medicine
基金
国家自然科学基金项目(81202918)
杭州市重大科技创新项目(20131813A23)
杭州市科技计划项目(20130533B68)
浙江中医药大学校级重点基金项目(2013ZZ12)
关键词
多指标
白芍
麸炒
指纹图谱
HPLC
multi index
Radix Paeoniae Alba
stir fried with bran
fingerprint
HPLC
