摘要
本文利用化学计量学交替三线性分解(ATLD)方法解析三维荧光数据,在有未知干扰组分共存的情况下,对中药升麻和人体血浆中升麻素的含量和回收率进行了测定,利用核一致诊断法判断得到的组分数是2。中药升麻和血浆体系的背景与升麻素有严重的重叠,用ATLD方法得到的解析光谱与真实光谱几乎完全重合,定量解析结果也令人满意。测定结果显示,标准曲线法和二阶标准加入法得到的中药升麻中升麻素的含量分别为0.061±0.003%和0.055±0.002%,回收率为89.8±4.7%,人体血浆中升麻素的回收率分别为102.7±1.6%。实验结果表明,此法可用于干扰组分共存下中药升麻和人体血浆中升麻素的快速准确定量分析。
A simple method for determination of Cimifugin in Cimicifugafoetida L. and human plasma even in the presence of other uncalibrated interferents by using excitation-emission matrix （EEM） fluorescence coupled with second-order calibration method based on alternating trilinear decomposition （ATLD） algorithm, the core consistency diagnostic （CORCONDIA） test indicated the correct number of components should be two. The EEM spectra of the analytes were overlapped with the background in Cimicifugafoetida L. and human plasma samples. The obtained results showed that the resolved spectra and real spectra of Cimifugin matched quite well with each other and the content of Cimifugin by standard curve and second-order standard addition method with component number N=2 are 0.061±0.003 % and 0.055±0.002 %, respectively. The average recovery is 89.8±4.7 %, and the average recovery in human plasma is 102.7±1.6 %. The experimental results show that the proposed method could be applied to simply quantify Cimifugin in Cimicifugafoetida L. and human plasma samples even in the presence of other uncalibrated interferents.
出处
《计算机与应用化学》
CAS
2015年第4期455-458,共4页
Computers and Applied Chemistry
基金
国家自然科学基金资助项目(中药三维荧光指纹图谱分析方法的标准化问题研究20975029
中药三维荧光检验法研究81173496)
关键词
交替三线性分解方法
三维荧光
升麻素
血浆
alternating trilinear decomposition method
excitation-emission matrix fluorescence
Cimifugin
human plasma
