摘要
目的研究中医复合因素造模法塑造脾虚型大鼠模型的效果,并探讨中医复合因素造模的特点和优势。方法采用中医传统病因:饥饱失常+劳倦+破气耗气方法塑造脾气虚型大鼠模型,将30只大鼠随机分为正常组15只,模型组15只,模型组予以每日灌服小承气汤溶液(剂量为2.5g/kg),隔日半量进食,每日下午将大鼠放入水深50cm水温20℃的水槽中游泳,以力竭为度(大鼠鼻尖没入水面10s),造模2周。正常组每日正常饮食活动,而后两组大鼠每日灌服生理盐水(剂量为5ml/kg),连续灌胃15d,通过观察大鼠的一般表现,体重、进食、饮水量、游泳力竭时间、胃排空、胃黏膜情况评价脾虚型大鼠模型的效果。结果造模后模型组大鼠的体重、进食、饮水量以及游泳力竭时间均下降,与正常组比较差异具有统计学意义,(P<0.05或0.01),经过灌胃生理盐水15d后,模型组大鼠与正常组大鼠在体重、进食、饮水量以及游泳力竭时间差异仍有统计学意义,且胃排空率明显低于正常组,(P<0.01)。结论中医复合因素能较快塑造脾虚型大鼠的证候,并且效果可维持,简便,价格低廉,更符合中医证候的内涵。
Objective To investigate the effects of composite factors make spleen - deficient model in rats and discuss the advantage. Methods Establish the rat model of spleen deficiency, 30 rats were divided randomly into two groups, the normal and the model. Model group was given xiaochengqi decoction （2.5 g/kg） daily mad half feed on alternate days. Put rats in the water to swim until exhaustion （ nasal tip under the water for 10s） , Building two weeks. Then two groups were given saline （5mL/kg） for 15 days. Observing the general performance, weight, eating, drinking, swimming exhaustion time, gastric emptying and gastric mucosa to evaluate the model of spleen deficient. Results The model group rats＂ weight, eating, drinking and swimming exhaustion time decreased compare with normal group, （P 〈 0.05or0.01 ） ; The model group also decreased compare with normal group after given saline for 15 days. And gastric emptying rate significantly reduced compare with normal group, （P 〈 0.01 ）. Conclusion Composite factors could quick shaping spleen deficient syndromes. And the effect can maintain, easy, cheap, more in accordance with the connotation of TCM syndrome.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2015年第4期1022-1024,共3页
Lishizhen Medicine and Materia Medica Research
基金
国家自然科学基金(No.81373564)
第五批全国名老中医专家学术传承工作项目
关键词
中医复合因素
脾虚
大鼠模型
Composite factors
Spleen deficiency
The model of rats
