摘要
b HLH类型转录因子对于植物开花具有重要的调控作用.为了揭示b HLH转录因子在短日照植物水稻中的功能,利用反向遗传学方法构建了水稻Osb HLH1基因过表达载体,获得过表达转基因植株,分析目的基因在过量表达条件下的功能.结果表明:过表达Osb HLH1能够导致水稻的抽穗期推迟;Osb HLH1在水稻中的表达还具有光周期昼夜节律性,无论是在长日照还是在短日照条件下,Osb HLH1都在夜间具有表达高峰.组织特异性表达结果表明:Osb HLH1在水稻的不同器官中都有表达,但在叶片中表达较高,与光周期相关基因的表达吻合.
The transcription factor bHLH plays a critical role in the regulation of plant flowering. To reveal the function of bHLH in rice,which is a short-day plant, an ectopic vector of OsbHLH1 gene was constructed and the transgenic plants were obtained by the reveme genetics method. The resuhs showed that the heading-date was delayed in ectopic transgenic plants, which suggested that OsbHLH1 can regulate the heading-date of flee. Further analysis showed that the expression of Os- bHLH1 displayed the 24 h-day-night rhythm expression and it had the peak in the night whether under long-day or short-day conditions. The analysis of expressive tissue-specificity demonstrated that OsbHLH1 displayed the expression in all organs in rice, especially had the higher expression in leaf, which was in consistent with the expression of photoperiod-specific genes.
出处
《天津师范大学学报（自然科学版）》
CAS
2015年第2期67-70,共4页
Journal of Tianjin Normal University：Natural Science Edition
基金
国家自然科学基金资助项目(31171515)
天津市高校中青年骨干创新人才培养计划资助项目(ZX110gg017)
