摘要
目的观察通阳逐瘀法对急性心肌缺血大鼠心肌组织IL-6的影响。方法选用SD大鼠64只,采用冠状动脉结扎法制备大鼠急性心肌缺血模型,随机分为假手术组、模型组、葱白提取物组、大黄提取物组、通阳逐瘀7∶3组、通阳逐瘀5∶5组、通阳逐瘀3∶7组和硝酸甘油组,以大鼠血清IL-6和心肌组织IL-6 mRNA为观测指标,观察通阳逐瘀法对急性心肌缺血大鼠炎症因子IL-6的干预作用。结果造模后大鼠血清IL-6含量升高明显(P<0.01);与模型组比较,各治疗组均能降低血清IL-6含量,通阳逐瘀7∶3组血清IL-6水平降低最为明显(P<0.01)。造模后大鼠心肌组织IL-6 mRNA明显表达(P<0.05),扩增倍数为1.00;经药物治疗后,IL-6 mRNA的表达明显降低,其中通阳逐瘀7∶3组抑制率最为显著。结论通阳逐瘀法能降低急性心肌缺血大鼠血清及心肌组织IL-6的表达水平。
Abstracts：Objective To study the effect of activating yang and removing stasis on the myocardial tissue of rats with acute myocardial ischemia IL -6.Methods 64 SD rats with acute myocardial ischemia rat model by coronary artery ligation method, were randomly divided into sham operation group, model group, fistular onion stalk extract group, rhubarb extract group, Yang Zhuyu group 7∶3, Yang Zhuyu group 5∶5, Yang Zhuyu group 3∶7 and nitroglycerin group, the serum IL -6 and myocardial tissues in rats with IL -6mRNA as observation indexes to observe intervention effect of activating yang and removing stasis on cytokines of IL -6 in rats with acute myocardial ischemia.Results After modeling, the content of IL -6 in serum of rats increased significantly （P 〈0.01）; compared with the model group, all the treatment group reduced serum IL -6 content, serum level of IL -6 in Yang stasis 7∶3 group was lower obviously （P 〈0.01）.After making the model of rat myocardial tissue IL -6mRNA expression espressed obviously （P 〈0.05）, amplification factor was 1; after drug treatment, the expres-sion of IL -6mRNA was decreased, the Yang stasis 7∶3 group was the most significant inhibitory rate.Conclusion Activating yang and blood stasis can reduce the expression levels of IL -6 in serum and myocardial tissue of rats with acute myocardial ischemia.
出处
《湖北中医药大学学报》
2015年第2期16-19,共4页
Journal of Hubei University of Chinese Medicine
基金
国家中医药管理局重点研究室建设项目(国中医药科技中医便函〔2012〕100号)
关键词
心肌缺血
通阳逐瘀法
白介素
-6
myocardial ischemia
activating yang and blood stasis
interleukin -6
