摘要
目的探讨100%氧对盲肠结扎穿孔(CLP)诱导的C57BL/6小鼠脓毒症的保护作用及NO介导的杀菌机制。方法首先采用完全随机设计的方法将雄性C57BL/6小鼠分为四组:假手术+空气组,假手术+100%氧组,盲肠结扎穿孔术+空气组,盲肠结扎穿孔术+100%氧组。小鼠在手术后6 h持续吸入100%氧2 h;相应的对照组自由呼吸空气。比较小鼠术后7d生存率(每组n=20),术后24 h腹腔灌洗液菌落计数(每组n=6),术后24 h腹腔灌洗液NO的产量(每组n=6)。然后另外将30只小鼠随机分为五组:假手术+生理盐水+空气组,盲肠结扎穿孔术+生理盐水+空气组,盲肠结扎穿孔术+LNAME+空气组,盲肠结扎穿孔术+生理盐水+100%氧组,盲肠结扎穿孔术+L-NAME+100%氧组,术后2 h给予腹腔注射L-NAME(20 mg/kg);相应对照组给予等体积的无菌生理盐水(NS)。比较各组术后24 h腹腔灌洗液菌落计数。结果与假手术+空气组比较,盲肠结扎穿孔术+空气组致死率及腹腔灌洗液的细菌计数均升高(均P<0.001),腹腔灌洗液中NO的产量增加(P<0.01);假手术+100%氧组各项指标与其比较差异无统计学意义(P>0.05)。与盲肠结扎穿孔术+空气组比较,盲肠结扎穿孔术+100%氧组生存率升高(P<0.05),腹腔灌洗液中脓毒症小鼠的细菌清除率上升(P<0.001),腹腔灌洗液中NO的含量升高(P<0.01)。假手术+100%氧组与假手术+空气组结果相似。与盲肠结扎穿孔术+NS+100%氧组比较,盲肠结扎穿孔术+L-NAME+100%氧组腹腔灌洗液中的菌落计数增加(P<0.01),盲肠结扎穿孔术+NS+空气组与盲肠结扎穿孔术+L-NAME+空气组比较腹腔灌洗液中的菌落计数没有统计学差异。结论 100%氧可能通过增加腹腔液中NO的产量来提高脓毒症小鼠的杀菌能力,从而改善CLP诱导的C57BL/6小鼠的脓毒症。
Objective To explore the protective effect of 100% oxygen（ Oxy） on cecal ligation and puncture（ CLP）-induced sepsis in C57 BL /6 mice and its NO-mediated bactericidal mechanism.Methods 1 The male C57 BL /6 mice were randomized into four groups：sham ＋ air group,sham ＋ 100% oxygen group,CLP ＋ air group and CLP ＋ 100% oxygen group.Mice were inhaled 100% Oxy for 2 h in experiment groups at 6 h after the operation,and mice inhaled air freely in control groups.The 7 d-survival rate,the number of colony forming units（ CFUs） in peritoneal lavage fluids at 24 h after operation and the production of NO in peritoneal lavage fluids at24 h after operation were compared among four groups.2Thirty male C57 BL /6 mice were randomized into five groups：sham ＋ NS ＋ air group,CLP ＋ NS ＋ air group,CLP ＋ L-NAME ＋ air group,CLP ＋ NS ＋ 100% Oxy group and CLP ＋ L-NAME ＋ 100% Oxy group.LNAME（ 20 mg / kg） was intraperitoneally injected at 2 h after the operation in experiment groups and normal saline was administered in the corresponding control groups.The number of CFUs in peritoneal lavage fluids at 24 h after operation.Results Compared with sham ＋ air group,the lethality and the CFU counts in peritoneal lavage fluids increased in CLP ＋ air group（ P〈 0.001）,and the production of NO was significantly increased in peritoneal lavage fluids（ P 〈0.01）,but there was no significant difference between sham ＋air group and sham ＋ 100% Oxy group.Compared with CLP ＋ air group,100% oxygen significantly improved the survival rate of septic mice（ P 〈0.05）,enhanced the bacterial clearance in peritoneal lavage fluids in septic mice（ P〈 0.001）,and further increased the content of NO in peritoneal lavage of septic mice（ P 〈0.01）,but there was no significant difference between sham ＋ 100% Oxy group and sham ＋ air group.After intraperitoneal injection of NO synthase inhibitor,compared with CLP ＋ 100% Oxy group,CFU counts in peritoneal lavage fluid were significantly
出处
《山西医科大学学报》
CAS
2015年第4期317-320,共4页
Journal of Shanxi Medical University
