摘要
目的探讨8-硝基白杨素(8-NOCHR)体外增强顺铂(DDP)诱导人卵巢癌HO-8910细胞凋亡的效应及其机制。方法采用MTT法检测8-NOCHR增强DDP对人卵巢癌HO-8910细胞增殖的影响,Western blot检测不同浓度8-NOCHR及DDP作用后HO-8910细胞P38MAPK和P-P38MAPK蛋白水平的表达情况;分光光度法分别检测8-NOCHR及DDP作用后HO-8910细胞GSH含量的变化以及Caspase-3活性的变化。结果 8-NOCHR能增强DDP对HO-8910细胞的诱导凋亡效果。各用药组P38MAPK、P-P38MAPK的表达均表现不同程度地增强(P<0.05),Caspase-3的活性表现不同程度增高(P<0.01),GSH含量不同程度降低(P<0.01)。结论 8-NOCHR能促进顺铂诱导HO-8910细胞的凋亡;8-NOCHR的化疗增敏作用与细胞内GSH的耗竭,Caspase-3活性增加,促进P38MAPK、P-P38MAPK蛋白表达有关。
Objective To explore the effect of 5,7-Dihydroxyl-8-Nitrio Chrysin（8-NOCHR）for enhancing the cisplatin（DDP）-induced apoptosis of human ovarian carcinoma HO-8910 cells in vitro and its possible mechanism. Methods MTI＇ assay was used to detect the proliferation of HO-8910 cells administrated by 8-NOChR combined with DDP. The expression levels of P38MAPK protein and P- P38 MAPK protein were determined by Western blot, and the content of GSH and the viability of Caspase-3 was analyzed by spectropho- tometic method. Results 8-NOCHR enhanced the effects of DDP on the apoptosis of H0-8910 cells in vitro. There was statistical difference in the expression levels of P38MAPK protein and P-P38MAPK protein after treated with different concentrations of 8-NOCHR combined with DDP （ P 〈 0.05 ）. The viability of caspase-3 protein was improved while the content of GSH was decreased in different degree after treated the different concentrations of 8-NOCHR combined with DDP （ P 〈 0.01 ）. Conclusion 8-NOChR could enhance the effects of DDP on the apoptosis of HO-8910 cells, which may be related to up-regulation of P38MAPK protein and P-P38MAPK protein, the increased viability of caspase-3 protein and the decrease of GSH.
出处
《山西医科大学学报》
CAS
2015年第4期309-313,共5页
Journal of Shanxi Medical University
关键词
8-硝基白杨素
顺铂
卵巢癌
细胞凋亡
5,7-Dihydroxyl-8-Nitrio Chrysin
cisplatin
ovarian cancer
cell apoptosis
