摘要
研究了纤维堆囊菌(Sorangium cellulosum)So F5-76在5 L发酵罐水平上发酵生产埃博霉素B的基本工艺参数,具体考察了接种量、搅拌转速、通气量、添加消泡剂及补糖等5个工艺参数对埃博霉素B发酵产量的影响。最后确定发酵罐基本发酵条件为接种量9%,搅拌转速180 r/min,空气流量3.5 L/min,消泡剂种类选择Antifoam B聚醚类消泡剂,补糖控制在发酵液糖浓度为0.2 g/L,在此条件下埃博霉素B的产量可达25.6 mg/L。
In order to improve the production of epothilone B,the effects of basic technological parameters of fermentation of Sorangium cellulosum So F5-76 were studied in a 5 L pilot fermenter,concretely inoculation volume,stir rmp,aeration rate,addition of defoaming agent,and sugar addition were investigated. Finally it was confirmed that basic fermentation condition of the fermenter were as follows： inoculation volume 9 %,stir rmp at 180,aeration rate at 3. 5 L / min,selected B polyether defoamer as defoaming agent,and sugar concentration in fermentation broth was controlled at 0. 2 g / L. Under these optimum fermentation conditions,the yield of epothilone B was as high as 25. 6 mg / L.
出处
《微生物学杂志》
CAS
CSCD
2015年第1期67-72,共6页
Journal of Microbiology
基金
国家自然科学基金资助项目(20906058)
陕西科技大学学术骨干培育项目(XSG2010009)
关键词
埃博霉素B
发酵罐
发酵条件
Sorangium cellulosum
epothilone B
fermenter
fermentation condition
