摘要
目的:研究复脉汤对急性心肌缺血大鼠肌钙蛋白Ⅰ(c TnⅠ)含量及心肌组织超微结构的影响。方法:将SD大鼠分为假手术组、模型组、炙甘草汤组、酒石酸美托洛尔组、复脉汤高、中、低剂量组,灌胃给药14 d后,结扎SD大鼠冠状动脉左前降支维持1 h(除假手术组外),制成急性心肌缺血模型,腹主动脉取血采用ELISA法检测血清c TnⅠ含量,电镜下观察各组大鼠心肌细胞的形态改变。结果:各剂量复脉汤、美托洛尔可不同程度降低c Tn I浓度,减轻心肌缺血导致的心肌细胞超微结构的损伤。与炙甘草汤比较,差异有统计学意义(P<0.05,P<0.01)。结论:复脉汤对结扎左冠状动脉造成的急性心肌缺血大鼠具有保护作用,其作用机制与降低外周血c TnⅠ水平及改善心肌超微结构损伤有关。
Objective：To study the effect of Fumai decoction on serum cardiac troponinⅠ（c TnⅠ）content and ultrastructure of cardiac muscle of rat models with acute myocardial ischemia. Methods：SD rats were randomly divided into sham operation group,model group,Zhigancao decoction group,metoprolol tartrate group,high,medium and low dosage groups of Fumai decoction. 14 days after the administration,rat models of acute myocardial ischemia were prepared,then concentration of c Tn I in the serum of rats was detected by ELISA. Morphological changes of myocardial cells were observed under electron microscope. Results：Compared with Zhigancao decoction group,concentration of c Tn Ⅰ was decreased and injury of myocardial cell was reduced in high and medium dosage groups of Fumai decoction and metoprolol tartrate group（P〈0.05,P〈0.01）. Conclusion：Fumai decoction had protective effect on rats with acute myocardial ischemia through reducing c TnⅠ level in the peripheral blood and improving myocardial ultrastructure.
出处
《山西中医学院学报》
2014年第6期15-17,共3页
Journal of Shanxi College of Traditional Chinese Medicine
基金
浙江省自然科学基金(LY12H27010)
关键词
复脉汤
急性心肌缺血
肌钙蛋白I
心肌超微结构
Fumai decoction
acute myocardial ischemia
cardiac troponinⅠ
ultrastructure of cardiac muscle
