摘要
为了筛选有抗肿瘤活性的银杏内生真菌及次生代谢产物,采用MTT法,用105株银杏内生真菌发酵液的乙酸乙酯粗提物分别对A549肺癌细胞与Hep G2肝癌细胞进行抗肿瘤活性的检测。结果显示:有12株内生真菌对A549细胞表现出一定的抑制,有11株内生真菌对Hep G2细胞表现出一定的抑制,经过梯度浓度筛选,其中菌株G-04-05对A549和Hep G2细胞都显现出了较高抑制作用,对其活性成分值得进一步的探索和研究。
To select the anti-tumor endophytic fungi and secondary metabolites,the anti-tumor effects of ethylacetate extracts of 105 endophytic fungi from Ginkgo on lung A549 cells and liver Hep G2 cells were detected by MTT assay. The results revealed that 12 strains of endophytic fungi showed inhibitory effects on lung A549 cells,and 11 strains demonstrated inhibitory effects on liver Hep G2 cells. In addition,the endophytic fungi strains G-04-05 was proved to be highly inhibitory to both A549 cells and Hep G2 cells by the concentration gradient screening. Therefore,the active components of this strain might be worth further investigation.
出处
《山地农业生物学报》
2015年第1期52-55,65,共5页
Journal of Mountain Agriculture and Biology
基金
贵州省国际科技合作计划项目
黔科合外G字[2012]7006
贵州省科学基金项目(黔科合丁字20112157)
贵州省科技计划项目(黔科合高G字20104009)
关键词
银杏内生真菌
抗肿瘤
抑制率
Endophytic fungi from Ginkgo
Anti-tumor
Inhibition ratio
